Efficient quantification of HIV-1 in heparin plasma spiked with cultured HIV-1 by the roche cobas taqman and abbott realtime HIV-1 tests

Linda L. Jagodzinski; Holly R. Weston; Ying Liu; Robert J. O'Connell; Sheila A. Peel

doi:10.1128/JCM.00706-12

Efficient quantification of HIV-1 in heparin plasma spiked with cultured HIV-1 by the roche cobas taqman and abbott realtime HIV-1 tests

Linda L. Jagodzinski^*, Holly R. Weston, Ying Liu, Robert J. O'Connell, Sheila A. Peel

^*Corresponding author for this work

Infectious Disease Clinical Research Program (IDCRP)

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The current automated real-time HIV-1 viral load assays, the Roche Cobas AmpliPrep/Cobas TaqMan test and the Abbott Real-Time test, are FDA cleared for use with EDTA plasma. We show that both real-time reverse transcription-PCR (RT-PCR) tests reliably quantify HIV-1 RNA in heparin plasma specimens spiked with HIV-1 isolate MN.

Original language	English
Pages (from-to)	2804-2806
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	50
Issue number	8
DOIs	https://doi.org/10.1128/JCM.00706-12
State	Published - Aug 2012

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abstract = "The current automated real-time HIV-1 viral load assays, the Roche Cobas AmpliPrep/Cobas TaqMan test and the Abbott Real-Time test, are FDA cleared for use with EDTA plasma. We show that both real-time reverse transcription-PCR (RT-PCR) tests reliably quantify HIV-1 RNA in heparin plasma specimens spiked with HIV-1 isolate MN.",

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AU - Liu, Ying

AU - O'Connell, Robert J.

AU - Peel, Sheila A.

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AB - The current automated real-time HIV-1 viral load assays, the Roche Cobas AmpliPrep/Cobas TaqMan test and the Abbott Real-Time test, are FDA cleared for use with EDTA plasma. We show that both real-time reverse transcription-PCR (RT-PCR) tests reliably quantify HIV-1 RNA in heparin plasma specimens spiked with HIV-1 isolate MN.

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Efficient quantification of HIV-1 in heparin plasma spiked with cultured HIV-1 by the roche cobas taqman and abbott realtime HIV-1 tests

Abstract

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