TY - JOUR
T1 - Electrochemical techniques to investigate adsorption and desorption behavior of fibrinogen on a gold surface
AU - Mohanraj, Gowtham
AU - Clark, Caelen M.
AU - Baboukani, Behnoosh Sattari
AU - Nalam, Prathima C.
AU - Ehrensberger, Mark T.
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2022/10
Y1 - 2022/10
N2 - Abstract: Fibrinogen interactions with cathodically polarized gold surfaces were characterized by electrochemical methods. Electrochemical Quartz Crystal Microbalance (eQCM) studies showed that fibrinogen desorption from gold surfaces occurred in a voltage-dependent manner. This study evaluated the cathodic-voltage-controlled electrical stimulation (CVCES) of gold. CVCES of − 1.5 V vs Ag/AgCl to the gold surface resulted in 97.9% desorption of the initially adsorbed protein, while − 1.2 V vs Ag/AgCl caused 63.2% desorption, and − 1 V vs Ag/AgCl produced only 5.6% desorption. Potentiodynamic and Scanning Electrochemical Microscopy (SECM) studies were conducted to investigate the mechanisms behind fibrinogen removal. It was determined that the water reduction reaction, which generates hydrogen, plays a key role in the removal of fibrinogen at − 1.2 V and − 1.5 V. Microenvironment surrounding gold during an electrical stimulation measured hydrogen evolution and pH change. Topographical images using Atomic Force Microscope (AFM) images, before and after the application of CVCES, confirmed the 63.2% and 97.9% removal of fibrinogen from the gold substrate at – 1.2 V and – 1.5 V, respectively. Graphical abstract: [Figure not available: see fulltext.]
AB - Abstract: Fibrinogen interactions with cathodically polarized gold surfaces were characterized by electrochemical methods. Electrochemical Quartz Crystal Microbalance (eQCM) studies showed that fibrinogen desorption from gold surfaces occurred in a voltage-dependent manner. This study evaluated the cathodic-voltage-controlled electrical stimulation (CVCES) of gold. CVCES of − 1.5 V vs Ag/AgCl to the gold surface resulted in 97.9% desorption of the initially adsorbed protein, while − 1.2 V vs Ag/AgCl caused 63.2% desorption, and − 1 V vs Ag/AgCl produced only 5.6% desorption. Potentiodynamic and Scanning Electrochemical Microscopy (SECM) studies were conducted to investigate the mechanisms behind fibrinogen removal. It was determined that the water reduction reaction, which generates hydrogen, plays a key role in the removal of fibrinogen at − 1.2 V and − 1.5 V. Microenvironment surrounding gold during an electrical stimulation measured hydrogen evolution and pH change. Topographical images using Atomic Force Microscope (AFM) images, before and after the application of CVCES, confirmed the 63.2% and 97.9% removal of fibrinogen from the gold substrate at – 1.2 V and – 1.5 V, respectively. Graphical abstract: [Figure not available: see fulltext.]
KW - Fibrinogen
KW - Gold-coated quartz crystal
KW - Hydrogen evolution
KW - Water reduction reaction
UR - http://www.scopus.com/inward/record.url?scp=85135335012&partnerID=8YFLogxK
U2 - 10.1007/s10800-022-01720-y
DO - 10.1007/s10800-022-01720-y
M3 - Article
AN - SCOPUS:85135335012
SN - 0021-891X
VL - 52
SP - 1525
EP - 1534
JO - Journal of Applied Electrochemistry
JF - Journal of Applied Electrochemistry
IS - 10
ER -