Electrochemical techniques to investigate adsorption and desorption behavior of fibrinogen on a gold surface

Gowtham Mohanraj, Caelen M. Clark, Behnoosh Sattari Baboukani, Prathima C. Nalam, Mark T. Ehrensberger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Abstract: Fibrinogen interactions with cathodically polarized gold surfaces were characterized by electrochemical methods. Electrochemical Quartz Crystal Microbalance (eQCM) studies showed that fibrinogen desorption from gold surfaces occurred in a voltage-dependent manner. This study evaluated the cathodic-voltage-controlled electrical stimulation (CVCES) of gold. CVCES of − 1.5 V vs Ag/AgCl to the gold surface resulted in 97.9% desorption of the initially adsorbed protein, while − 1.2 V vs Ag/AgCl caused 63.2% desorption, and − 1 V vs Ag/AgCl produced only 5.6% desorption. Potentiodynamic and Scanning Electrochemical Microscopy (SECM) studies were conducted to investigate the mechanisms behind fibrinogen removal. It was determined that the water reduction reaction, which generates hydrogen, plays a key role in the removal of fibrinogen at − 1.2 V and − 1.5 V. Microenvironment surrounding gold during an electrical stimulation measured hydrogen evolution and pH change. Topographical images using Atomic Force Microscope (AFM) images, before and after the application of CVCES, confirmed the 63.2% and 97.9% removal of fibrinogen from the gold substrate at – 1.2 V and – 1.5 V, respectively. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1525-1534
Number of pages10
JournalJournal of Applied Electrochemistry
Issue number10
StatePublished - Oct 2022
Externally publishedYes


  • Fibrinogen
  • Gold-coated quartz crystal
  • Hydrogen evolution
  • Water reduction reaction


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