Electrochemical techniques to investigate adsorption and desorption behavior of fibrinogen on a gold surface

Gowtham Mohanraj; Caelen M. Clark; Behnoosh Sattari Baboukani; Prathima C. Nalam; Mark T. Ehrensberger

doi:10.1007/s10800-022-01720-y

Electrochemical techniques to investigate adsorption and desorption behavior of fibrinogen on a gold surface

Gowtham Mohanraj, Caelen M. Clark, Behnoosh Sattari Baboukani, Prathima C. Nalam, Mark T. Ehrensberger^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Abstract: Fibrinogen interactions with cathodically polarized gold surfaces were characterized by electrochemical methods. Electrochemical Quartz Crystal Microbalance (eQCM) studies showed that fibrinogen desorption from gold surfaces occurred in a voltage-dependent manner. This study evaluated the cathodic-voltage-controlled electrical stimulation (CVCES) of gold. CVCES of − 1.5 V vs Ag/AgCl to the gold surface resulted in 97.9% desorption of the initially adsorbed protein, while − 1.2 V vs Ag/AgCl caused 63.2% desorption, and − 1 V vs Ag/AgCl produced only 5.6% desorption. Potentiodynamic and Scanning Electrochemical Microscopy (SECM) studies were conducted to investigate the mechanisms behind fibrinogen removal. It was determined that the water reduction reaction, which generates hydrogen, plays a key role in the removal of fibrinogen at − 1.2 V and − 1.5 V. Microenvironment surrounding gold during an electrical stimulation measured hydrogen evolution and pH change. Topographical images using Atomic Force Microscope (AFM) images, before and after the application of CVCES, confirmed the 63.2% and 97.9% removal of fibrinogen from the gold substrate at – 1.2 V and – 1.5 V, respectively. Graphical abstract: [Figure not available: see fulltext.]

Original language	English
Pages (from-to)	1525-1534
Number of pages	10
Journal	Journal of Applied Electrochemistry
Volume	52
Issue number	10
DOIs	https://doi.org/10.1007/s10800-022-01720-y
State	Published - Oct 2022
Externally published	Yes

Keywords

Fibrinogen
Gold-coated quartz crystal
Hydrogen evolution
Water reduction reaction

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10.1007/s10800-022-01720-y

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@article{847db3c9fca248388618790d6581192e,

title = "Electrochemical techniques to investigate adsorption and desorption behavior of fibrinogen on a gold surface",

abstract = "Abstract: Fibrinogen interactions with cathodically polarized gold surfaces were characterized by electrochemical methods. Electrochemical Quartz Crystal Microbalance (eQCM) studies showed that fibrinogen desorption from gold surfaces occurred in a voltage-dependent manner. This study evaluated the cathodic-voltage-controlled electrical stimulation (CVCES) of gold. CVCES of − 1.5 V vs Ag/AgCl to the gold surface resulted in 97.9% desorption of the initially adsorbed protein, while − 1.2 V vs Ag/AgCl caused 63.2% desorption, and − 1 V vs Ag/AgCl produced only 5.6% desorption. Potentiodynamic and Scanning Electrochemical Microscopy (SECM) studies were conducted to investigate the mechanisms behind fibrinogen removal. It was determined that the water reduction reaction, which generates hydrogen, plays a key role in the removal of fibrinogen at − 1.2 V and − 1.5 V. Microenvironment surrounding gold during an electrical stimulation measured hydrogen evolution and pH change. Topographical images using Atomic Force Microscope (AFM) images, before and after the application of CVCES, confirmed the 63.2% and 97.9% removal of fibrinogen from the gold substrate at – 1.2 V and – 1.5 V, respectively. Graphical abstract: [Figure not available: see fulltext.]",

keywords = "Fibrinogen, Gold-coated quartz crystal, Hydrogen evolution, Water reduction reaction",

author = "Gowtham Mohanraj and Clark, {Caelen M.} and Baboukani, {Behnoosh Sattari} and Nalam, {Prathima C.} and Ehrensberger, {Mark T.}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature B.V.",

year = "2022",

month = oct,

doi = "10.1007/s10800-022-01720-y",

language = "English",

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journal = "Journal of Applied Electrochemistry",

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TY - JOUR

T1 - Electrochemical techniques to investigate adsorption and desorption behavior of fibrinogen on a gold surface

AU - Mohanraj, Gowtham

AU - Clark, Caelen M.

AU - Baboukani, Behnoosh Sattari

AU - Nalam, Prathima C.

AU - Ehrensberger, Mark T.

PY - 2022/10

Y1 - 2022/10

N2 - Abstract: Fibrinogen interactions with cathodically polarized gold surfaces were characterized by electrochemical methods. Electrochemical Quartz Crystal Microbalance (eQCM) studies showed that fibrinogen desorption from gold surfaces occurred in a voltage-dependent manner. This study evaluated the cathodic-voltage-controlled electrical stimulation (CVCES) of gold. CVCES of − 1.5 V vs Ag/AgCl to the gold surface resulted in 97.9% desorption of the initially adsorbed protein, while − 1.2 V vs Ag/AgCl caused 63.2% desorption, and − 1 V vs Ag/AgCl produced only 5.6% desorption. Potentiodynamic and Scanning Electrochemical Microscopy (SECM) studies were conducted to investigate the mechanisms behind fibrinogen removal. It was determined that the water reduction reaction, which generates hydrogen, plays a key role in the removal of fibrinogen at − 1.2 V and − 1.5 V. Microenvironment surrounding gold during an electrical stimulation measured hydrogen evolution and pH change. Topographical images using Atomic Force Microscope (AFM) images, before and after the application of CVCES, confirmed the 63.2% and 97.9% removal of fibrinogen from the gold substrate at – 1.2 V and – 1.5 V, respectively. Graphical abstract: [Figure not available: see fulltext.]

AB - Abstract: Fibrinogen interactions with cathodically polarized gold surfaces were characterized by electrochemical methods. Electrochemical Quartz Crystal Microbalance (eQCM) studies showed that fibrinogen desorption from gold surfaces occurred in a voltage-dependent manner. This study evaluated the cathodic-voltage-controlled electrical stimulation (CVCES) of gold. CVCES of − 1.5 V vs Ag/AgCl to the gold surface resulted in 97.9% desorption of the initially adsorbed protein, while − 1.2 V vs Ag/AgCl caused 63.2% desorption, and − 1 V vs Ag/AgCl produced only 5.6% desorption. Potentiodynamic and Scanning Electrochemical Microscopy (SECM) studies were conducted to investigate the mechanisms behind fibrinogen removal. It was determined that the water reduction reaction, which generates hydrogen, plays a key role in the removal of fibrinogen at − 1.2 V and − 1.5 V. Microenvironment surrounding gold during an electrical stimulation measured hydrogen evolution and pH change. Topographical images using Atomic Force Microscope (AFM) images, before and after the application of CVCES, confirmed the 63.2% and 97.9% removal of fibrinogen from the gold substrate at – 1.2 V and – 1.5 V, respectively. Graphical abstract: [Figure not available: see fulltext.]

KW - Fibrinogen

KW - Gold-coated quartz crystal

KW - Hydrogen evolution

KW - Water reduction reaction

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DO - 10.1007/s10800-022-01720-y

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JO - Journal of Applied Electrochemistry

JF - Journal of Applied Electrochemistry

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ER -

Electrochemical techniques to investigate adsorption and desorption behavior of fibrinogen on a gold surface

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Keywords

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