ELISA underestimates measles antibody seroprevalence in US military recruits

James D. Mancuso; Margot R. Krauss; Susette Audet; Judy A. Beeler

doi:10.1016/j.vaccine.2008.06.028

ELISA underestimates measles antibody seroprevalence in US military recruits

James D. Mancuso^*, Margot R. Krauss, Susette Audet, Judy A. Beeler

^*Corresponding author for this work

Research output: Contribution to journal › Letter › peer-review

15 Scopus citations

Abstract

The prevalence of antibodies to measles, mumps, and rubella in US military recruits is of importance to public health leaders. We performed ELISA testing using a commercially available product on samples from 537 recruits obtained in 1998, of which 437 were positive (81%). We then performed a validation study in a subsample of the population using plaque reduction neutralization (PRN) to assess misclassification error. This resulted in a corrected estimate of the prevalence of immunity to measles of 96% (95% CI: 92-100%). The military vaccinates a percentage of recruits who are likely to be immune if more sensitive testing, such as PRN, was used.

Original language	English
Pages (from-to)	4877-4878
Number of pages	2
Journal	Vaccine
Volume	26
Issue number	38
DOIs	https://doi.org/10.1016/j.vaccine.2008.06.028
State	Published - 8 Sep 2008
Externally published	Yes

Keywords

Immunizations
Measles epidemiology
Military personnel
Seroepidemiology

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title = "ELISA underestimates measles antibody seroprevalence in US military recruits",

abstract = "The prevalence of antibodies to measles, mumps, and rubella in US military recruits is of importance to public health leaders. We performed ELISA testing using a commercially available product on samples from 537 recruits obtained in 1998, of which 437 were positive (81%). We then performed a validation study in a subsample of the population using plaque reduction neutralization (PRN) to assess misclassification error. This resulted in a corrected estimate of the prevalence of immunity to measles of 96% (95% CI: 92-100%). The military vaccinates a percentage of recruits who are likely to be immune if more sensitive testing, such as PRN, was used.",

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author = "Mancuso, {James D.} and Krauss, {Margot R.} and Susette Audet and Beeler, {Judy A.}",

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AU - Mancuso, James D.

AU - Krauss, Margot R.

AU - Audet, Susette

AU - Beeler, Judy A.

PY - 2008/9/8

Y1 - 2008/9/8

N2 - The prevalence of antibodies to measles, mumps, and rubella in US military recruits is of importance to public health leaders. We performed ELISA testing using a commercially available product on samples from 537 recruits obtained in 1998, of which 437 were positive (81%). We then performed a validation study in a subsample of the population using plaque reduction neutralization (PRN) to assess misclassification error. This resulted in a corrected estimate of the prevalence of immunity to measles of 96% (95% CI: 92-100%). The military vaccinates a percentage of recruits who are likely to be immune if more sensitive testing, such as PRN, was used.

AB - The prevalence of antibodies to measles, mumps, and rubella in US military recruits is of importance to public health leaders. We performed ELISA testing using a commercially available product on samples from 537 recruits obtained in 1998, of which 437 were positive (81%). We then performed a validation study in a subsample of the population using plaque reduction neutralization (PRN) to assess misclassification error. This resulted in a corrected estimate of the prevalence of immunity to measles of 96% (95% CI: 92-100%). The military vaccinates a percentage of recruits who are likely to be immune if more sensitive testing, such as PRN, was used.

KW - Immunizations

KW - Measles epidemiology

KW - Military personnel

KW - Seroepidemiology

UR - http://www.scopus.com/inward/record.url?scp=50049104448&partnerID=8YFLogxK

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M3 - Letter

C2 - 18586062

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SN - 0264-410X

VL - 26

SP - 4877

EP - 4878

JO - Vaccine

JF - Vaccine

IS - 38

ER -

ELISA underestimates measles antibody seroprevalence in US military recruits

Abstract

Keywords

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