ELISA underestimates measles antibody seroprevalence in US military recruits

James D. Mancuso*, Margot R. Krauss, Susette Audet, Judy A. Beeler

*Corresponding author for this work

Research output: Contribution to journalLetterpeer-review

15 Scopus citations


The prevalence of antibodies to measles, mumps, and rubella in US military recruits is of importance to public health leaders. We performed ELISA testing using a commercially available product on samples from 537 recruits obtained in 1998, of which 437 were positive (81%). We then performed a validation study in a subsample of the population using plaque reduction neutralization (PRN) to assess misclassification error. This resulted in a corrected estimate of the prevalence of immunity to measles of 96% (95% CI: 92-100%). The military vaccinates a percentage of recruits who are likely to be immune if more sensitive testing, such as PRN, was used.

Original languageEnglish
Pages (from-to)4877-4878
Number of pages2
Issue number38
StatePublished - 8 Sep 2008
Externally publishedYes


  • Immunizations
  • Measles epidemiology
  • Military personnel
  • Seroepidemiology


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