Enhancement of the chemiluminescence response of enzymatic reactions by plasmonic surfaces for biosensing applications

Biebele Abel, Babatunde Odukoya, Muzaffer Mohammed, Kadir Aslan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We report the enhancement of chemiluminescence response of horseradish peroxidase (HRP) in bioassays by plasmonic surfaces, which are comprised of (i) silver island films (SIFs) and (ii) metal thin films (silver, gold, copper, and nickel, 1 nm thick) deposited onto glass slides. A model bioassay, based on the interactions of avidin-modified HRP with a monolayer of biotinylated poly(ethylene-glycol)-amine, was employed to evaluate the ability of plasmonic surfaces to enhance chemiluminescence response of HRP. Chemiluminescence response of HRP in model bioassays were increased up to ~3.7-fold as compared to the control samples (i.e. glass slides without plasmonic nanoparticles), where the largest enhancement of the chemiluminescence response was observed on SIFs with high loading. These findings allowed us to demonstrate the use of SIFs (high loading) for the detection of a biologically relevant target protein (glial fibrillary acidic protein or GFAP), where the chemiluminescence response of the standard bioassay for GFAP was enhanced up to ~50% as compared to bioassay on glass slides.

Original languageEnglish
Pages (from-to)92-101
Number of pages10
JournalNano Biomedicine and Engineering
Volume7
Issue number3
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • Amplification of chemiluminescence response
  • Glial fibrillary acidic protein
  • Horse radish peroxidase
  • Metal-enhanced chemiluminescence
  • Metal-enhanced fluorescence
  • Silver island films

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