Enrichment of low-molecular-weight proteins from biofluids for biomarker discovery

Oleg Chertov*, John T. Simpson, Arya Biragyn, Thomas P. Conrads, Timothy D. Veenstra, Robert J. Fisher

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

51 Scopus citations


The dramatic progress in mass spectrometry-based methods of protein identification has triggered a new quest for disease-associated biomarkers. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and its variant surface-enhanced laser desorption/ionization mass spectrometry, provide effective means to explore the less studied information slice of the human serum proteome - low-molecular-weight proteins and peptides. These low-molecular-weight proteins and peptides are promising for the detection of important biomarkers. Due to the significant experimental problems imposed by high-abundance and high-molecular-weight proteins, it is important to effectively remove these species prior to mass spectrometry analysis of the low-molecular-weight serum and plasma proteomes. In this review, the advantages afforded by recently introduced methods for prefractionation of serum, as they pertain to the detection and identification of biomarkers, will be discussed.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalExpert Review of Proteomics
Issue number1
StatePublished - Feb 2005
Externally publishedYes


  • Biomarkers
  • Cancer
  • Early diagnosis
  • Mass spectrometry
  • Plasma
  • Prefractionation
  • Serum


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