Epimorphic regeneration approach to tissue replacement in adult mammals

Vineet Agrawal, Scott A. Johnson, Janet Reing, Li Zhang, Stephen Tottey, Gang Wang, Karen K. Hirschi, Susan Braunhut, Lorraine J. Gudas, Stephen F. Badylak

Research output: Contribution to journalArticlepeer-review

131 Scopus citations


Urodeles and fetal mammals are capable of impressive epimorphic regeneration in a variety of tissues, whereas the typical default response to injury in adult mammals consists of inflammation and scar tissue formation. One component of epimorphic regeneration is the recruitment of resident progenitor and stem cells to a site of injury. Bioactive molecules resulting from degradation of extracellular matrix (ECM) have been shown to recruit a variety of progenitor and stem cells in vitro in adult mammals. The ability to recruit multipotential cells to the site of injury by in vivo administration of chemotactic ECM degradation products in a mammalian model of digit amputation was investigated in the present study. Adult, 6- to 8-week-old C57/BL6 mice were subjected to midsecond phalanx amputation of the third digit of the right hind foot and either treated with chemotactic ECM degradation products or left untreated. At 14 days after amputation, mice treated with ECM degradation products showed an accumulation of heterogeneous cells that expressed markers of multipotency, including Sox2, Sca1, and Rex1 (Zfp42). Cells isolated from the site of amputation were capable of differentiation along neuroectodermal and mesodermal lineages, whereas cells isolated from control mice were capable of differentiation along only mesodermal lineages. The present findings demonstrate the recruitment of endogenous stem cells to a site of injury, and/or their generation/ proliferation therein, in response to ECM degradation products.

Original languageEnglish
Pages (from-to)3351-3355
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number8
StatePublished - 23 Feb 2010
Externally publishedYes


  • Extracellular matrix
  • Microenvironment
  • Stem cell
  • Tissue engineering


Dive into the research topics of 'Epimorphic regeneration approach to tissue replacement in adult mammals'. Together they form a unique fingerprint.

Cite this