Escape from self-tolerance leads to neonatal insulin-dependent diabetes mellitus

Dorel L. Radu, Teodor Doru Brumeanu, Robert C. McEvoy, Constantin A. Bona*, Sofia Casares

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Double transgenic (dTg) mice expressing the hemagglutinin (HA) of influenza virus under the insulin promoter and the TCR specific for the immunodominant CD4 T cell epitope of HA (HA110-120) develop insulin-dependent diabetes mellitus (IDDM). In order to gain information on the breaking down of neonatal self-tolerance we studied the occurrence of IDDM after birth. Our results showed that newborn mice develop fulminant IDDM characterized by occurrence of insulitis as early as 3 days after birth, followed by hyperglycemia by 7 days, and significant hypoinsulinemia by 28 days. The neonatal breakdown of self-tolerance of T cells positively selected in the thymus is supported by the facts that: (i) peripheral HA110-120 specific T cells from neonates are fully functional and proliferated upon stimulation with the nominal peptide, and (ii) peptide-specific T cells were accumulated in the pancreas of dTg mice as early as 3 days after birth. Our results demonstrate that diabetes occurring in young dTg mice is due to early activation of self-reactive T cells immediately after birth. Accumulation of specific T cells in the target organ leads to destruction of pancreatic p-cells and IDDM. These mice may provide a useful model to evaluate new strategies for the prevention of diabetes.

Original languageEnglish
Pages (from-to)199-207
Number of pages9
Issue number4
StatePublished - 1999
Externally publishedYes


  • Hemagglutinin
  • IDDM
  • Neonatal tolerance
  • TCR


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