Evaluation of a Rapid Quantitative Diagnostic Test for Adenovirus Type 4

Dennis J. Faix; Huo Shu H. Houng; Joel C. Gaydos; Shih Kang S. Liu; John T. Connors; Xiomara Brown; Ludmila V. Asher; David W. Vaughn; Leonard N. Binn

doi:10.1086/380972

Evaluation of a Rapid Quantitative Diagnostic Test for Adenovirus Type 4

Dennis J. Faix, Huo Shu H. Houng, Joel C. Gaydos, Shih Kang S. Liu, John T. Connors, Xiomara Brown, Ludmila V. Asher, David W. Vaughn, Leonard N. Binn^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Acute respiratory disease (ARD) due to adenoviruses is a reemerging disease in military recruits. It is a challenge for clinicians to accurately diagnose this disease and to appropriately treat affected individuals. This study investigated the utility of a quantitative, rapid-cycle, real-time fluorogenic polymerase chain reaction (PCR) technique for detecting adenovirus type 4 (Ad4) in a clinical setting. Throat swab specimens and clinical data were collected from US Army basic trainees hospitalized with ARD at Fort Jackson, South Carolina. A total of 140 throat swab specimens were collected from 83 subjects. Rapid PCR results (obtained in <2 h) had a sensitivity of 100% and a specificity of 100%, compared with viral culture. There was no difference, qualitative or quantitative, between frozen and fresh samples for PCR detection of Ad4. Individuals with test results positive for Ad4 were hospitalized longer than were individuals with negative test results. Higher virus loads at hospital admission corresponded to longer lengths of stay for Ad4-positive subjects.

Original language	English
Pages (from-to)	391-397
Number of pages	7
Journal	Clinical Infectious Diseases
Volume	38
Issue number	3
DOIs	https://doi.org/10.1086/380972
State	Published - 1 Feb 2004
Externally published	Yes

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title = "Evaluation of a Rapid Quantitative Diagnostic Test for Adenovirus Type 4",

abstract = "Acute respiratory disease (ARD) due to adenoviruses is a reemerging disease in military recruits. It is a challenge for clinicians to accurately diagnose this disease and to appropriately treat affected individuals. This study investigated the utility of a quantitative, rapid-cycle, real-time fluorogenic polymerase chain reaction (PCR) technique for detecting adenovirus type 4 (Ad4) in a clinical setting. Throat swab specimens and clinical data were collected from US Army basic trainees hospitalized with ARD at Fort Jackson, South Carolina. A total of 140 throat swab specimens were collected from 83 subjects. Rapid PCR results (obtained in <2 h) had a sensitivity of 100% and a specificity of 100%, compared with viral culture. There was no difference, qualitative or quantitative, between frozen and fresh samples for PCR detection of Ad4. Individuals with test results positive for Ad4 were hospitalized longer than were individuals with negative test results. Higher virus loads at hospital admission corresponded to longer lengths of stay for Ad4-positive subjects.",

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AU - Connors, John T.

AU - Brown, Xiomara

AU - Asher, Ludmila V.

AU - Vaughn, David W.

AU - Binn, Leonard N.

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AB - Acute respiratory disease (ARD) due to adenoviruses is a reemerging disease in military recruits. It is a challenge for clinicians to accurately diagnose this disease and to appropriately treat affected individuals. This study investigated the utility of a quantitative, rapid-cycle, real-time fluorogenic polymerase chain reaction (PCR) technique for detecting adenovirus type 4 (Ad4) in a clinical setting. Throat swab specimens and clinical data were collected from US Army basic trainees hospitalized with ARD at Fort Jackson, South Carolina. A total of 140 throat swab specimens were collected from 83 subjects. Rapid PCR results (obtained in <2 h) had a sensitivity of 100% and a specificity of 100%, compared with viral culture. There was no difference, qualitative or quantitative, between frozen and fresh samples for PCR detection of Ad4. Individuals with test results positive for Ad4 were hospitalized longer than were individuals with negative test results. Higher virus loads at hospital admission corresponded to longer lengths of stay for Ad4-positive subjects.

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Evaluation of a Rapid Quantitative Diagnostic Test for Adenovirus Type 4

Abstract

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