Evaluation of antibiotic releasing porous polymethylmethacrylate space maintainers in an infected composite tissue defect model

Patrick P. Spicer, Sarita R. Shah, Allan M. Henslee, Brendan M. Watson, Lucas A. Kinard, James D. Kretlow, Kristin Bevil, Lauren Kattchee, George N. Bennett, Nagi Demian, Katrin Mende, Clinton K. Murray, John A. Jansen, Mark E. Wong, Antonios G. Mikos, F. Kurtis Kasper*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


This study evaluated the in vitro and in vivo performance of antibiotic-releasing porous polymethylmethacrylate (PMMA)-based space maintainers comprising a gelatin hydrogel porogen and a poly(dl-lactic-co- glycolic acid) (PLGA) particulate carrier for antibiotic delivery. Colistin was released in vitro from either gelatin or PLGA microparticle loaded PMMA constructs, with gelatin-loaded constructs releasing colistin over approximately 7 days and PLGA microparticle-loaded constructs releasing colistin for up to 8 weeks. Three formulations with either burst release or extended release at different doses were tested in a rabbit mandibular defect inoculated with Acinetobacter baumannii (2 × 107 colony forming units ml -1). In addition, one material control that released antibiotic but was not inoculated with A. baumannii was tested. A. baumannii was not detectable in any animal after 12 weeks on culture of the defect, saliva, or blood. Defects with high dose extended release implants had greater soft tissue healing compared with defects with burst release implants, with 8 of 10 animals showing healed mucosae compared with 2 of 10 respectively. Extended release of locally delivered colistin via a PLGA microparticle carrier improved soft tissue healing compared with implants with burst release of colistin from a gelatin carrier.

Original languageEnglish
Pages (from-to)8832-8839
Number of pages8
JournalActa Biomaterialia
Issue number11
StatePublished - Nov 2013


  • Antibiotic delivery
  • Composite tissue defect
  • Craniofacial reconstruction
  • Infected defect
  • Space maintainer


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