Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys

Patrick J. Blair; Tadeusz J. Kochel; Kanakatte Raviprakash; Carolina Guevara; Milagros Salazar; Shuenn Jue Wu; James G. Olson; Kevin R. Porter

doi:10.1016/j.vaccine.2005.09.032

Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys

Patrick J. Blair, Tadeusz J. Kochel, Kanakatte Raviprakash, Carolina Guevara, Milagros Salazar, Shuenn Jue Wu, James G. Olson, Kevin R. Porter^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine.

Original language	English
Pages (from-to)	1427-1432
Number of pages	6
Journal	Vaccine
Volume	24
Issue number	9
DOIs	https://doi.org/10.1016/j.vaccine.2005.09.032
State	Published - 27 Feb 2006
Externally published	Yes

Keywords

Aotus monkeys
DNA vaccine
Dengue

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10.1016/j.vaccine.2005.09.032

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title = "Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys",

abstract = "A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine.",

keywords = "Aotus monkeys, DNA vaccine, Dengue",

author = "Blair, {Patrick J.} and Kochel, {Tadeusz J.} and Kanakatte Raviprakash and Carolina Guevara and Milagros Salazar and Wu, {Shuenn Jue} and Olson, {James G.} and Porter, {Kevin R.}",

note = "Funding Information: Funding: This work was funded by the Department of Defense Military Infectious Diseases Research Program, grant AS0021_02_LI (NMRC Work Unit 63002A 810S).",

year = "2006",

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doi = "10.1016/j.vaccine.2005.09.032",

language = "English",

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journal = "Vaccine",

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T1 - Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys

AU - Blair, Patrick J.

AU - Kochel, Tadeusz J.

AU - Raviprakash, Kanakatte

AU - Guevara, Carolina

AU - Salazar, Milagros

AU - Wu, Shuenn Jue

AU - Olson, James G.

AU - Porter, Kevin R.

N1 - Funding Information: Funding: This work was funded by the Department of Defense Military Infectious Diseases Research Program, grant AS0021_02_LI (NMRC Work Unit 63002A 810S).

PY - 2006/2/27

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N2 - A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine.

AB - A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine.

KW - Aotus monkeys

KW - DNA vaccine

KW - Dengue

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JO - Vaccine

JF - Vaccine

IS - 9

ER -

Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys

Abstract

Keywords

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