TY - JOUR
T1 - Exploring human plasma proteome strategies
T2 - High efficiency in-solution digestion protocol for multi-dimensional protein identification technology
AU - Ru, Qinhua Cindy
AU - Zhu, Luwang Andy
AU - Katenhusen, Richard A.
AU - Silberman, Jordan
AU - Brzeski, Henry
AU - Liebman, Michael
AU - Shriver, Craig D.
N1 - Funding Information:
This work was supported by Clinical Breast Care Project (CBCP). CBCP and Windber Research Institute are funded by the U.S. Department of Defense through the Henry Jackson Foundation for the Advancement of Military Medicine.
PY - 2006/4/14
Y1 - 2006/4/14
N2 - Multi-dimensional protein identification technology (MudPIT) is becoming a prevalent proteomic approach due to its high-throughput separations and accurate mass detection. Prior to MudPIT analysis, complicated samples required in-solution digestion. Unlike in-gel digestion, in which enzymes work on just a few proteins, in-solution digestion involves simultaneous digestion of hundreds or thousands of proteins. In-solution digestion protocols must therefore be very efficient. Few investigations have evaluated the efficiency of in-solution digestion protocols. The present research compared three such protocols. Results suggest that a protocol utilizing trifluoroethanol (TFE) as denaturant is most efficient.
AB - Multi-dimensional protein identification technology (MudPIT) is becoming a prevalent proteomic approach due to its high-throughput separations and accurate mass detection. Prior to MudPIT analysis, complicated samples required in-solution digestion. Unlike in-gel digestion, in which enzymes work on just a few proteins, in-solution digestion involves simultaneous digestion of hundreds or thousands of proteins. In-solution digestion protocols must therefore be very efficient. Few investigations have evaluated the efficiency of in-solution digestion protocols. The present research compared three such protocols. Results suggest that a protocol utilizing trifluoroethanol (TFE) as denaturant is most efficient.
KW - Clinical proteomics
KW - Human plasma
KW - Human plasma proteome
KW - In-solution digestion
KW - Multi-dimensional protein identification technology
UR - http://www.scopus.com/inward/record.url?scp=33645213674&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2005.06.080
DO - 10.1016/j.chroma.2005.06.080
M3 - Article
C2 - 16569577
AN - SCOPUS:33645213674
SN - 0021-9673
VL - 1111
SP - 175
EP - 191
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -