Extensively cross-reactive anti-HIV-1 neutralizing antibodies induced by gp140 immunization

Fei Zhang Peng; Fatim Cham; Ming Dong; Anil Choudhary; Peter Bouma; Zhiqiang Zhang; Yiming Shao; Yan Ru Feng; Lemin Wang; Nathalie Mathy; Gerald Voss; Christopher C. Broder; Gerald V. Quinnan

doi:10.1073/pnas.0608635104

Extensively cross-reactive anti-HIV-1 neutralizing antibodies induced by gp140 immunization

Fei Zhang Peng, Fatim Cham, Ming Dong, Anil Choudhary, Peter Bouma, Zhiqiang Zhang, Yiming Shao, Yan Ru Feng, Lemin Wang, Nathalie Mathy, Gerald Voss, Christopher C. Broder, Gerald V. Quinnan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

An immunization regimen was evaluated in rabbits consisting of the soluble, oligomeric form of envelope glycoprotein of HIV-1, strain R2 (gp140 _R2), or the surface component of the same envelope (Env), gp120 _R2, in the adjuvant AS02A. The gp140_R2 was selected based on its unusual CD4-independent phenotype and the exceptionally broad neutralizing response in the infected donor. The gp140_R2 immunogen induced antibodies that achieved 50% neutralization of 48/48, and 80% neutralization of 43/46 primary strains of diverse HIV-1 subtypes tested. The strains tested included members of standard panels of subtype B and C strains, and other diverse strains known to be neutralization resistant. The gp120 _R2 induced antibodies that neutralized 9/48 of the same strains. Neutralization was IgG-mediated and HIV-1-specific. These results demonstrate that induction of truly broad spectrum neutralizing antibodies is an achievable goal in HIV-1 vaccine development.

Original language	English
Pages (from-to)	10193-10198
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	104
Issue number	24
DOIs	https://doi.org/10.1073/pnas.0608635104
State	Published - 12 Jun 2007
Externally published	Yes

Keywords

Adjuvant
Envelope
Glycoprotein
Neutralization

Access to Document

10.1073/pnas.0608635104

Cite this

Peng, F. Z., Cham, F., Dong, M., Choudhary, A., Bouma, P., Zhang, Z., Shao, Y., Feng, Y. R., Wang, L., Mathy, N., Voss, G., Broder, C. C., & Quinnan, G. V. (2007). Extensively cross-reactive anti-HIV-1 neutralizing antibodies induced by gp140 immunization. Proceedings of the National Academy of Sciences of the United States of America, 104(24), 10193-10198. https://doi.org/10.1073/pnas.0608635104

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title = "Extensively cross-reactive anti-HIV-1 neutralizing antibodies induced by gp140 immunization",

abstract = "An immunization regimen was evaluated in rabbits consisting of the soluble, oligomeric form of envelope glycoprotein of HIV-1, strain R2 (gp140 R2), or the surface component of the same envelope (Env), gp120 R2, in the adjuvant AS02A. The gp140R2 was selected based on its unusual CD4-independent phenotype and the exceptionally broad neutralizing response in the infected donor. The gp140R2 immunogen induced antibodies that achieved 50% neutralization of 48/48, and 80% neutralization of 43/46 primary strains of diverse HIV-1 subtypes tested. The strains tested included members of standard panels of subtype B and C strains, and other diverse strains known to be neutralization resistant. The gp120 R2 induced antibodies that neutralized 9/48 of the same strains. Neutralization was IgG-mediated and HIV-1-specific. These results demonstrate that induction of truly broad spectrum neutralizing antibodies is an achievable goal in HIV-1 vaccine development.",

keywords = "Adjuvant, Envelope, Glycoprotein, Neutralization",

author = "Peng, {Fei Zhang} and Fatim Cham and Ming Dong and Anil Choudhary and Peter Bouma and Zhiqiang Zhang and Yiming Shao and Feng, {Yan Ru} and Lemin Wang and Nathalie Mathy and Gerald Voss and Broder, {Christopher C.} and Quinnan, {Gerald V.}",

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doi = "10.1073/pnas.0608635104",

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Peng, FZ, Cham, F, Dong, M, Choudhary, A, Bouma, P, Zhang, Z, Shao, Y, Feng, YR, Wang, L, Mathy, N, Voss, G, Broder, CC & Quinnan, GV 2007, 'Extensively cross-reactive anti-HIV-1 neutralizing antibodies induced by gp140 immunization', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 24, pp. 10193-10198. https://doi.org/10.1073/pnas.0608635104

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AU - Peng, Fei Zhang

AU - Cham, Fatim

AU - Dong, Ming

AU - Choudhary, Anil

AU - Bouma, Peter

AU - Zhang, Zhiqiang

AU - Shao, Yiming

AU - Feng, Yan Ru

AU - Wang, Lemin

AU - Mathy, Nathalie

AU - Voss, Gerald

AU - Broder, Christopher C.

AU - Quinnan, Gerald V.

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AB - An immunization regimen was evaluated in rabbits consisting of the soluble, oligomeric form of envelope glycoprotein of HIV-1, strain R2 (gp140 R2), or the surface component of the same envelope (Env), gp120 R2, in the adjuvant AS02A. The gp140R2 was selected based on its unusual CD4-independent phenotype and the exceptionally broad neutralizing response in the infected donor. The gp140R2 immunogen induced antibodies that achieved 50% neutralization of 48/48, and 80% neutralization of 43/46 primary strains of diverse HIV-1 subtypes tested. The strains tested included members of standard panels of subtype B and C strains, and other diverse strains known to be neutralization resistant. The gp120 R2 induced antibodies that neutralized 9/48 of the same strains. Neutralization was IgG-mediated and HIV-1-specific. These results demonstrate that induction of truly broad spectrum neutralizing antibodies is an achievable goal in HIV-1 vaccine development.

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