Fabrication and characterization of bioactive and antibacterial composites for dental applications

Xanthippi Chatzistavrou*, J. Christopher Fenno, Denver Faulk, Stephen Badylak, Toshihiro Kasuga, Aldo R. Boccaccini, Petros Papagerakis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

98 Scopus citations


There is an increasing clinical need to design novel dental materials that combine regenerative and antibacterial properties. In this work the characterization of a recently developed sol-gel-derived bioactive glass ceramic containing silver ions (Ag-BG) is presented. The microstructural characteristics, ion release profile, zeta potential value and changes in weight loss and pH value as a function of the immersion time of Ag-BG in Tris buffer are evaluated. Ag-BG is also incorporated into natural extracellular matrix (ECM) hydrogel to further enhance its regenerative properties. Then, the micro and macro architectures of these new composites (ECM/Ag-BG) are characterized. In addition, the antibacterial properties of these new composites are tested against Escherichia coli and Enterococcus faecalis, a bacterium commonly implicated in the pathogenesis of dental pulp infections. Cell-material interaction is also monitored in a primary culture of dental pulp cells. Our study highlights the benefits of the successful incorporation of Ag in the bioactive glass, resulting in a stable antibacterial material with long-lasting bactericidal activity. Furthermore, this work presents for the first time the fabrication of new Ag-doped composite materials, with inductive pulp-cell proliferation and antibacterial properties (ECM/Ag-BG). This advanced composite made of Ag-BG incorporated into natural ECM possesses improved properties that may facilitate potential applications in tooth regeneration approaches.

Original languageEnglish
Pages (from-to)3723-3732
Number of pages10
JournalActa Biomaterialia
Issue number8
StatePublished - Aug 2014
Externally publishedYes


  • Antibacterial action
  • Bioactive glass
  • Composite
  • Natural extracellular matrix scaffold
  • Silver


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