TY - JOUR
T1 - Fabrication and characterization of bioactive and antibacterial composites for dental applications
AU - Chatzistavrou, Xanthippi
AU - Fenno, J. Christopher
AU - Faulk, Denver
AU - Badylak, Stephen
AU - Kasuga, Toshihiro
AU - Boccaccini, Aldo R.
AU - Papagerakis, Petros
N1 - Funding Information:
Part of the presented research was funded by the Japan Society for the Promotion of Science (JSPS) in the framework of a JSPS fellowship as well as funding sources of Dr Papagerakis start-up funds. The authors wish to thank the group of Prof. Kasuga at NIT for their help on the implementation of the characterization work.
PY - 2014/8
Y1 - 2014/8
N2 - There is an increasing clinical need to design novel dental materials that combine regenerative and antibacterial properties. In this work the characterization of a recently developed sol-gel-derived bioactive glass ceramic containing silver ions (Ag-BG) is presented. The microstructural characteristics, ion release profile, zeta potential value and changes in weight loss and pH value as a function of the immersion time of Ag-BG in Tris buffer are evaluated. Ag-BG is also incorporated into natural extracellular matrix (ECM) hydrogel to further enhance its regenerative properties. Then, the micro and macro architectures of these new composites (ECM/Ag-BG) are characterized. In addition, the antibacterial properties of these new composites are tested against Escherichia coli and Enterococcus faecalis, a bacterium commonly implicated in the pathogenesis of dental pulp infections. Cell-material interaction is also monitored in a primary culture of dental pulp cells. Our study highlights the benefits of the successful incorporation of Ag in the bioactive glass, resulting in a stable antibacterial material with long-lasting bactericidal activity. Furthermore, this work presents for the first time the fabrication of new Ag-doped composite materials, with inductive pulp-cell proliferation and antibacterial properties (ECM/Ag-BG). This advanced composite made of Ag-BG incorporated into natural ECM possesses improved properties that may facilitate potential applications in tooth regeneration approaches.
AB - There is an increasing clinical need to design novel dental materials that combine regenerative and antibacterial properties. In this work the characterization of a recently developed sol-gel-derived bioactive glass ceramic containing silver ions (Ag-BG) is presented. The microstructural characteristics, ion release profile, zeta potential value and changes in weight loss and pH value as a function of the immersion time of Ag-BG in Tris buffer are evaluated. Ag-BG is also incorporated into natural extracellular matrix (ECM) hydrogel to further enhance its regenerative properties. Then, the micro and macro architectures of these new composites (ECM/Ag-BG) are characterized. In addition, the antibacterial properties of these new composites are tested against Escherichia coli and Enterococcus faecalis, a bacterium commonly implicated in the pathogenesis of dental pulp infections. Cell-material interaction is also monitored in a primary culture of dental pulp cells. Our study highlights the benefits of the successful incorporation of Ag in the bioactive glass, resulting in a stable antibacterial material with long-lasting bactericidal activity. Furthermore, this work presents for the first time the fabrication of new Ag-doped composite materials, with inductive pulp-cell proliferation and antibacterial properties (ECM/Ag-BG). This advanced composite made of Ag-BG incorporated into natural ECM possesses improved properties that may facilitate potential applications in tooth regeneration approaches.
KW - Antibacterial action
KW - Bioactive glass
KW - Composite
KW - Natural extracellular matrix scaffold
KW - Silver
UR - http://www.scopus.com/inward/record.url?scp=84903732359&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2014.04.030
DO - 10.1016/j.actbio.2014.04.030
M3 - Article
C2 - 24802300
AN - SCOPUS:84903732359
SN - 1742-7061
VL - 10
SP - 3723
EP - 3732
JO - Acta Biomaterialia
JF - Acta Biomaterialia
IS - 8
ER -