TY - JOUR
T1 - Frequent overexpression of ETS-related gene-1 (ERG1) in prostate cancer transcriptome
AU - Petrovics, Gyorgy
AU - Liu, Aijun
AU - Shaheduzzaman, Syed
AU - Furasato, Bungo
AU - Sun, Chen
AU - Chen, Yongmei
AU - Nau, Martin
AU - Ravindranath, Lakshmi
AU - Chen, Yidong
AU - Dobi, Albert
AU - Srikantan, Vasantha
AU - Sesterhenn, Isabell A.
AU - McLeod, David G.
AU - Vahey, Maryanne
AU - Moul, Judd W.
AU - Srivastava, Shiv
N1 - Funding Information:
The prostate tissue specimens used in this study were obtained under an IRB-approved protocol at Walter Reed Army Medical Center. Informed consent was obtained from each subject. We thank DK Watson for providing ERG1 and ERG2 cDNA clones and for critically reading the manuscript. This work was funded by the CPDR through an ongoing grant from the US Army Medical Research and Materiel Command, and by NIH Grant RO1 DK065977 to SS. None of the authors have competing financial interests. The opinions and assertions contained herein are the private views of the authors and are not to be construed as reflecting the official views of the US Army or the Department of Defense.
PY - 2005/5/26
Y1 - 2005/5/26
N2 - Transcription factors encoded by the ETS family of genes are central in integrating signals that regulate cell growth and differentiation, stress responses, and tumorigenesis. This study, analysing laser microdissected paired benign and malignant prostate epithelial cells from prostate cancer (CaP) patients (n = 114; 228 specimen) by GeneChip and quantitative real-time RT-PCR, identifies ETS-related gene (ERG), a member of the ETS transcription factor family, as the most frequently overexpressed proto-oncogene in the transcriptome of malignant prostate epithelial cells. Combined quantitative expression analysis of ERG with two other genes commonly overexpressed in CaP, AMACR and DD3, revealed overexpression of at least one of these three genes in virtually all CaP specimen (54 of 55). Comprehensive evaluation of quantitative ERG1 expression with clinicopathological features also suggested that ERG1 expression level in prostate tumor cells relative to benign epithelial cells is indicator of disease-free survival after radical prostatectomy.
AB - Transcription factors encoded by the ETS family of genes are central in integrating signals that regulate cell growth and differentiation, stress responses, and tumorigenesis. This study, analysing laser microdissected paired benign and malignant prostate epithelial cells from prostate cancer (CaP) patients (n = 114; 228 specimen) by GeneChip and quantitative real-time RT-PCR, identifies ETS-related gene (ERG), a member of the ETS transcription factor family, as the most frequently overexpressed proto-oncogene in the transcriptome of malignant prostate epithelial cells. Combined quantitative expression analysis of ERG with two other genes commonly overexpressed in CaP, AMACR and DD3, revealed overexpression of at least one of these three genes in virtually all CaP specimen (54 of 55). Comprehensive evaluation of quantitative ERG1 expression with clinicopathological features also suggested that ERG1 expression level in prostate tumor cells relative to benign epithelial cells is indicator of disease-free survival after radical prostatectomy.
KW - ERG1
KW - ETS transcription factor family
KW - Overexpression
KW - Prostate cancer
KW - Proto-oncogene
UR - http://www.scopus.com/inward/record.url?scp=20444493600&partnerID=8YFLogxK
U2 - 10.1038/sj.onc.1208518
DO - 10.1038/sj.onc.1208518
M3 - Article
C2 - 15750627
AN - SCOPUS:20444493600
SN - 0950-9232
VL - 24
SP - 3847
EP - 3852
JO - Oncogene
JF - Oncogene
IS - 23
ER -