Functional characterization of ex vivo blood myeloid and plasmacytoid dendritic cells after infection with dengue virus

Peifang Sun; Stefan Fernandez; Mary A. Marovich; Dupeh R. Palmer; Christina M. Celluzzi; Kobporn Boonnak; Zhaodong Liang; Hemavathy Subramanian; Kevin R. Porter; Wellington Sun; Timothy H. Burgess

doi:10.1016/j.virol.2008.10.022

Functional characterization of ex vivo blood myeloid and plasmacytoid dendritic cells after infection with dengue virus

Peifang Sun^*, Stefan Fernandez, Mary A. Marovich, Dupeh R. Palmer, Christina M. Celluzzi, Kobporn Boonnak, Zhaodong Liang, Hemavathy Subramanian, Kevin R. Porter, Wellington Sun, Timothy H. Burgess

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

Myeloid and plasmacytoid dendritic cells (mDC and pDC) are naturally distinctive subsets. We exposed both subsets to dengue virus (DV) in vitro and investigated their functional characteristics. High levels of DV replication in mDC were found to correlate with DC-SIGN expression. Production of inflammatory cytokines by mDC increased gradually after DV-infection, which was dependent on DV replication. Co-stimulatory markers were upregulated on mDC upon DV-infection. On the contrary, lower levels of DV-replication were observed in pDC, but the cytokine production in pDC was quicker and stronger. This cytokine response was not dependent on viral replication, but dependent on cell endosomal activity and TLR7, and could be also induced by purified DV genome RNA. These results clearly suggested functional differences between mDC and pDC in response to DV infection. Additionally, the TLR7-mediated recognition of DV RNA may be involved in pDC functional activation.

Original language	English
Pages (from-to)	207-215
Number of pages	9
Journal	Virology
Volume	383
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.10.022
State	Published - 20 Jan 2009

Keywords

Cytokine
Dendritic cell subset
Dengue virus
Toll-like receptor
Viral infection

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10.1016/j.virol.2008.10.022

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title = "Functional characterization of ex vivo blood myeloid and plasmacytoid dendritic cells after infection with dengue virus",

abstract = "Myeloid and plasmacytoid dendritic cells (mDC and pDC) are naturally distinctive subsets. We exposed both subsets to dengue virus (DV) in vitro and investigated their functional characteristics. High levels of DV replication in mDC were found to correlate with DC-SIGN expression. Production of inflammatory cytokines by mDC increased gradually after DV-infection, which was dependent on DV replication. Co-stimulatory markers were upregulated on mDC upon DV-infection. On the contrary, lower levels of DV-replication were observed in pDC, but the cytokine production in pDC was quicker and stronger. This cytokine response was not dependent on viral replication, but dependent on cell endosomal activity and TLR7, and could be also induced by purified DV genome RNA. These results clearly suggested functional differences between mDC and pDC in response to DV infection. Additionally, the TLR7-mediated recognition of DV RNA may be involved in pDC functional activation.",

keywords = "Cytokine, Dendritic cell subset, Dengue virus, Toll-like receptor, Viral infection",

author = "Peifang Sun and Stefan Fernandez and Marovich, {Mary A.} and Palmer, {Dupeh R.} and Celluzzi, {Christina M.} and Kobporn Boonnak and Zhaodong Liang and Hemavathy Subramanian and Porter, {Kevin R.} and Wellington Sun and Burgess, {Timothy H.}",

note = "Funding Information: This work was supported by U.S. Navy work unit number 61102A.870.S.A0015. The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government. ",

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doi = "10.1016/j.virol.2008.10.022",

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AU - Sun, Peifang

AU - Fernandez, Stefan

AU - Marovich, Mary A.

AU - Palmer, Dupeh R.

AU - Celluzzi, Christina M.

AU - Boonnak, Kobporn

AU - Liang, Zhaodong

AU - Subramanian, Hemavathy

AU - Porter, Kevin R.

AU - Sun, Wellington

AU - Burgess, Timothy H.

N1 - Funding Information: This work was supported by U.S. Navy work unit number 61102A.870.S.A0015. The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government.

PY - 2009/1/20

Y1 - 2009/1/20

N2 - Myeloid and plasmacytoid dendritic cells (mDC and pDC) are naturally distinctive subsets. We exposed both subsets to dengue virus (DV) in vitro and investigated their functional characteristics. High levels of DV replication in mDC were found to correlate with DC-SIGN expression. Production of inflammatory cytokines by mDC increased gradually after DV-infection, which was dependent on DV replication. Co-stimulatory markers were upregulated on mDC upon DV-infection. On the contrary, lower levels of DV-replication were observed in pDC, but the cytokine production in pDC was quicker and stronger. This cytokine response was not dependent on viral replication, but dependent on cell endosomal activity and TLR7, and could be also induced by purified DV genome RNA. These results clearly suggested functional differences between mDC and pDC in response to DV infection. Additionally, the TLR7-mediated recognition of DV RNA may be involved in pDC functional activation.

AB - Myeloid and plasmacytoid dendritic cells (mDC and pDC) are naturally distinctive subsets. We exposed both subsets to dengue virus (DV) in vitro and investigated their functional characteristics. High levels of DV replication in mDC were found to correlate with DC-SIGN expression. Production of inflammatory cytokines by mDC increased gradually after DV-infection, which was dependent on DV replication. Co-stimulatory markers were upregulated on mDC upon DV-infection. On the contrary, lower levels of DV-replication were observed in pDC, but the cytokine production in pDC was quicker and stronger. This cytokine response was not dependent on viral replication, but dependent on cell endosomal activity and TLR7, and could be also induced by purified DV genome RNA. These results clearly suggested functional differences between mDC and pDC in response to DV infection. Additionally, the TLR7-mediated recognition of DV RNA may be involved in pDC functional activation.

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KW - Dendritic cell subset

KW - Dengue virus

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Functional characterization of ex vivo blood myeloid and plasmacytoid dendritic cells after infection with dengue virus

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Keywords

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