Functional interplay between the cell cycle and cell phenotypes

Wei Chiang Chen, Pei Hsun Wu, Jude M. Phillip, Shyam B. Khatau, Jae Min Choi, Matthew R. Dallas, Konstantinos Konstantopoulos, Sean X. Sun, Jerry S.H. Lee, Didier Hodzic, Denis Wirtz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Cell cycle distribution of adherent cells is typically assessed using flow cytometry, which precludes the measurements of many cell properties and their cycle phase in the same environment. Here we develop and validate a microscopy system to quantitatively analyze the cell-cycle phase of thousands of adherent cells and their associated cell properties simultaneously. This assay demonstrates that population-averaged cell phenotypes can be written as a linear combination of cell-cycle fractions and phase-dependent phenotypes. By perturbing the cell cycle through inhibition of cell-cycle regulators or changing nuclear morphology by depletion of structural proteins, our results reveal that cell cycle regulators and structural proteins can significantly interfere with each other's prima facie functions. This study introduces a high-throughput method to simultaneously measure the cell cycle and phenotypes at single-cell resolution, which reveals a complex functional interplay between the cell cycle and cell phenotypes.

Original languageEnglish
Pages (from-to)523-534
Number of pages12
JournalIntegrative Biology
Volume5
Issue number3
DOIs
StatePublished - Mar 2013
Externally publishedYes

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