Gender differences in Na/Ca exchanger current and β-adrenergic responsiveness in heart failure in pig myocytes

Shao Kui Wei, John M. Mccurley, Stephen U. Hanlon, Mark C.P. Haigney*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

21 Scopus citations

Abstract

Clinical trials suggest females experience less heart failure (HF) progression, mortality, and arrhythmia frequency. HF increases Na/Ca exchanger (NCX) expression and activity contributing to both depressed contractility and ventricular arrhythmias, but whether gender modifies this effect is unknown. Left ventricular myocytes were isolated from control and from tachycardic pacing-induced failing swine hearts of both sexes. The Ni-sensitive NCX current (INCX) was measured in voltage clamp after blocking other channels. In control myocytes there is no difference in basal INCX and β-adrenergic responsiveness between male and female animals. HF greatly increased INCX and reduced β-adrenergic responsiveness in males compared to females, an effect that was eliminated by PP1. Diuretic therapy (furosemide, 1 mg/kg/day) further enhanced INCX and reduced β-adrenergic responsiveness in females and eliminated the gender difference. Gender-specific differences in calcium handling may contribute to improved survival of females in HF.

Original languageEnglish
Title of host publicationSodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion
Subtitle of host publicationFifth International Conference
PublisherBlackwell Publishing Inc.
Pages183-189
Number of pages7
ISBN (Print)1573316490, 9781573316491
DOIs
StatePublished - Mar 2007
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1099
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Gender
  • Heart failure
  • Phosphorylation
  • Sodium-calcium exchange

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