Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia

Kevin R. Porter*, John R. Mascola, Hudoyo Hupudio, Daniel Ewing, Thomas C. VanCott, Ronald L. Anthony, Andrew L. Corwin, Soeprapto Widodo, Soekardjo Ertono, Francine E. McCutchan, Donald S. Burke, Curtis G. Hayes, F. Stephen Wignall, Robert R. Graham

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Issue number1
StatePublished - 1 Jan 1997
Externally publishedYes


  • Genotype
  • HIV-1
  • Neutralization tests


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