Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia

Kevin R. Porter; John R. Mascola; Hudoyo Hupudio; Daniel Ewing; Thomas C. VanCott; Ronald L. Anthony; Andrew L. Corwin; Soeprapto Widodo; Soekardjo Ertono; Francine E. McCutchan; Donald S. Burke; Curtis G. Hayes; F. Stephen Wignall; Robert R. Graham

doi:10.1097/00042560-199701010-00001

Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia

Kevin R. Porter^*
, John R. Mascola
, Hudoyo Hupudio
, Daniel Ewing
, Thomas C. VanCott
, Ronald L. Anthony
, Andrew L. Corwin
, Soeprapto Widodo
, Soekardjo Ertono
, Francine E. McCutchan
, Donald S. Burke
, Curtis G. Hayes
, F. Stephen Wignall
, Robert R. Graham

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1097/00042560-199701010-00001
State	Published - 1 Jan 1997
Externally published	Yes

Keywords

Genotype
HIV-1
Neutralization tests

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10.1097/00042560-199701010-00001

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Porter, K. R., Mascola, J. R., Hupudio, H., Ewing, D., VanCott, T. C., Anthony, R. L., Corwin, A. L., Widodo, S., Ertono, S., McCutchan, F. E., Burke, D. S., Hayes, C. G., Wignall, F. S., & Graham, R. R. (1997). Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 14(1), 1-6. https://doi.org/10.1097/00042560-199701010-00001

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title = "Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia",

abstract = "To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.",

keywords = "Genotype, HIV-1, Neutralization tests",

author = "Porter, \{Kevin R.\} and Mascola, \{John R.\} and Hudoyo Hupudio and Daniel Ewing and VanCott, \{Thomas C.\} and Anthony, \{Ronald L.\} and Corwin, \{Andrew L.\} and Soeprapto Widodo and Soekardjo Ertono and McCutchan, \{Francine E.\} and Burke, \{Donald S.\} and Hayes, \{Curtis G.\} and Wignall, \{F. Stephen\} and Graham, \{Robert R.\}",

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doi = "10.1097/00042560-199701010-00001",

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Porter, KR, Mascola, JR, Hupudio, H, Ewing, D, VanCott, TC, Anthony, RL, Corwin, AL, Widodo, S, Ertono, S, McCutchan, FE, Burke, DS, Hayes, CG, Wignall, FS & Graham, RR 1997, 'Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia', Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, vol. 14, no. 1, pp. 1-6. https://doi.org/10.1097/00042560-199701010-00001

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AU - Anthony, Ronald L.

AU - Corwin, Andrew L.

AU - Widodo, Soeprapto

AU - Ertono, Soekardjo

AU - McCutchan, Francine E.

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N2 - To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.

AB - To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.

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JF - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology

IS - 1

ER -

Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia

Abstract

Keywords

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