TY - JOUR
T1 - Genetic Engineering of T Cells for Immune Tolerance
AU - Scott, David W.
N1 - Publisher Copyright:
© 2019
PY - 2020/3/13
Y1 - 2020/3/13
N2 - Regulatory T cells (Tregs) play a role in the induction and maintenance of tolerance, as well as in modulating aberrant immune responses. While expanded Tregs have been used in clinical trials, they are polyclonal and the frequency of specific Tregs is very low. To overcome this issue, we have endeavored to “specify” Tregs by engineering them to express receptors that can recognize a given antigen and applied this protocol in autoimmunity, hemophilia and allergy. Thus, we have used retroviral transduction of a specific T cell receptor, single-chain variable fragments (Fvs), or antigen domains in Tregs to achieve this goal. This review summarizes our steps to achieve the ultimate goal of modulating human diseases.
AB - Regulatory T cells (Tregs) play a role in the induction and maintenance of tolerance, as well as in modulating aberrant immune responses. While expanded Tregs have been used in clinical trials, they are polyclonal and the frequency of specific Tregs is very low. To overcome this issue, we have endeavored to “specify” Tregs by engineering them to express receptors that can recognize a given antigen and applied this protocol in autoimmunity, hemophilia and allergy. Thus, we have used retroviral transduction of a specific T cell receptor, single-chain variable fragments (Fvs), or antigen domains in Tregs to achieve this goal. This review summarizes our steps to achieve the ultimate goal of modulating human diseases.
UR - http://www.scopus.com/inward/record.url?scp=85077460120&partnerID=8YFLogxK
U2 - 10.1016/j.omtm.2019.11.022
DO - 10.1016/j.omtm.2019.11.022
M3 - Review article
AN - SCOPUS:85077460120
SN - 2329-0501
VL - 16
SP - 103
EP - 107
JO - Molecular Therapy - Methods and Clinical Development
JF - Molecular Therapy - Methods and Clinical Development
ER -