Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle

Karine G. Le Roch; Jeffrey R. Johnson; Laurence Florens; Yingyao Zhou; Andrey Santrosyan; Munira Grainger; S. Frank Yan; Kim C. Williamson; Anthony A. Holder; Daniel J. Carucci; John R. Yates; Elizabeth A. Winzeler

doi:10.1101/gr.2523904

Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle

Karine G. Le Roch, Jeffrey R. Johnson, Laurence Florens, Yingyao Zhou, Andrey Santrosyan, Munira Grainger, S. Frank Yan, Kim C. Williamson, Anthony A. Holder, Daniel J. Carucci, John R. Yates, Elizabeth A. Winzeler^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

380 Scopus citations

Abstract

To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.

Original language	English
Pages (from-to)	2308-2318
Number of pages	11
Journal	Genome Research
Volume	14
Issue number	11
DOIs	https://doi.org/10.1101/gr.2523904
State	Published - Nov 2004
Externally published	Yes

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10.1101/gr.2523904

Cite this

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title = "Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle",

abstract = "To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.",

author = "{Le Roch}, {Karine G.} and Johnson, {Jeffrey R.} and Laurence Florens and Yingyao Zhou and Andrey Santrosyan and Munira Grainger and Yan, {S. Frank} and Williamson, {Kim C.} and Holder, {Anthony A.} and Carucci, {Daniel J.} and Yates, {John R.} and Winzeler, {Elizabeth A.}",

year = "2004",

month = nov,

doi = "10.1101/gr.2523904",

language = "English",

volume = "14",

pages = "2308--2318",

journal = "Genome Research",

issn = "1088-9051",

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TY - JOUR

T1 - Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle

AU - Le Roch, Karine G.

AU - Johnson, Jeffrey R.

AU - Florens, Laurence

AU - Zhou, Yingyao

AU - Santrosyan, Andrey

AU - Grainger, Munira

AU - Yan, S. Frank

AU - Williamson, Kim C.

AU - Holder, Anthony A.

AU - Carucci, Daniel J.

AU - Yates, John R.

AU - Winzeler, Elizabeth A.

PY - 2004/11

Y1 - 2004/11

N2 - To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.

AB - To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.

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U2 - 10.1101/gr.2523904

DO - 10.1101/gr.2523904

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SP - 2308

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JO - Genome Research

JF - Genome Research

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