Global expression profiling reveals a role for CTGF/CCN2 in lactogenic differentiation of mouse mammary epithelial cells

Weihan Wang; Cynthia Jose; Nicholas Kenney; Bethanie Morrison; Mary Lou Cutler

doi:10.1007/978-90-481-3779-4_12

Global expression profiling reveals a role for CTGF/CCN2 in lactogenic differentiation of mouse mammary epithelial cells

Weihan Wang, Cynthia Jose, Nicholas Kenney, Bethanie Morrison, Mary Lou Cutler^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Mammary epithelial cells undergo a series of developmental changes during pregnancy and lactation including proliferation, differentiation, secretion and apoptosis. HC11 mouse mammary epithelial cells, which are capable of lactogen-induced differentiation in cell culture, were used to follow the changes in gene expression during this process. The expression profiles of over 20,000 genes were compared in HC11 cells undergoing lactogenic differentiation to non-differentiated cells using DNA microarray analysis. Greater than two fold changes were detected in 998 genes in the differentiated cells versus growth controls. Several genes, including CTGF/CCN2, exhibited greater than five-fold increase. Validation of the gene expression changes verified the involvement of numerous genes and pathways in the differentiation of mouse mammary epithelial cells and identified genetic pathways associated with specific transcriptional regulation. Further analysis revealed EGF-dependent regulation of a specific subset of genes including important cell cycle regulators. The expression of a subset of genes regulated by lactogenic differentiation in HC11 cells, including CTGF/CCN2 and osteopontin, was examined in mouse mammary glands revealing in vivo changes in expression during pregnancy and lactation. In addition, elevation or depletion of CTGF/CCN2 in HC11 cells had a significant effect on the degree of lactogenic differentiation observed in the cells. The studies confirm the value of expression profiling in defining transcriptional controls associated with differentiation of mammary epithelial cells and revealed novel regulators of the process, including CTGF/CCN2.

Original language	English
Title of host publication	CCN Proteins in Health and Disease
Subtitle of host publication	An Overview of the Fifth International Workshop on the CCN Family of Genes
Publisher	Springer Netherlands
Pages	141-162
Number of pages	22
ISBN (Print)	9789048137787
DOIs	https://doi.org/10.1007/978-90-481-3779-4_12
State	Published - 2010
Externally published	Yes

Keywords

CTGF/CCN2
Dexamethasone
Lactogenic differentiation

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10.1007/978-90-481-3779-4_12

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Wang, W., Jose, C., Kenney, N., Morrison, B., & Cutler, M. L. (2010). Global expression profiling reveals a role for CTGF/CCN2 in lactogenic differentiation of mouse mammary epithelial cells. In CCN Proteins in Health and Disease: An Overview of the Fifth International Workshop on the CCN Family of Genes (pp. 141-162). Springer Netherlands. https://doi.org/10.1007/978-90-481-3779-4_12

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title = "Global expression profiling reveals a role for CTGF/CCN2 in lactogenic differentiation of mouse mammary epithelial cells",

abstract = "Mammary epithelial cells undergo a series of developmental changes during pregnancy and lactation including proliferation, differentiation, secretion and apoptosis. HC11 mouse mammary epithelial cells, which are capable of lactogen-induced differentiation in cell culture, were used to follow the changes in gene expression during this process. The expression profiles of over 20,000 genes were compared in HC11 cells undergoing lactogenic differentiation to non-differentiated cells using DNA microarray analysis. Greater than two fold changes were detected in 998 genes in the differentiated cells versus growth controls. Several genes, including CTGF/CCN2, exhibited greater than five-fold increase. Validation of the gene expression changes verified the involvement of numerous genes and pathways in the differentiation of mouse mammary epithelial cells and identified genetic pathways associated with specific transcriptional regulation. Further analysis revealed EGF-dependent regulation of a specific subset of genes including important cell cycle regulators. The expression of a subset of genes regulated by lactogenic differentiation in HC11 cells, including CTGF/CCN2 and osteopontin, was examined in mouse mammary glands revealing in vivo changes in expression during pregnancy and lactation. In addition, elevation or depletion of CTGF/CCN2 in HC11 cells had a significant effect on the degree of lactogenic differentiation observed in the cells. The studies confirm the value of expression profiling in defining transcriptional controls associated with differentiation of mammary epithelial cells and revealed novel regulators of the process, including CTGF/CCN2.",

keywords = "CTGF/CCN2, Dexamethasone, Lactogenic differentiation",

author = "Weihan Wang and Cynthia Jose and Nicholas Kenney and Bethanie Morrison and Cutler, {Mary Lou}",

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Wang, W, Jose, C, Kenney, N, Morrison, B & Cutler, ML 2010, Global expression profiling reveals a role for CTGF/CCN2 in lactogenic differentiation of mouse mammary epithelial cells. in CCN Proteins in Health and Disease: An Overview of the Fifth International Workshop on the CCN Family of Genes. Springer Netherlands, pp. 141-162. https://doi.org/10.1007/978-90-481-3779-4_12

Global expression profiling reveals a role for CTGF/CCN2 in lactogenic differentiation of mouse mammary epithelial cells. / Wang, Weihan; Jose, Cynthia; Kenney, Nicholas et al.
CCN Proteins in Health and Disease: An Overview of the Fifth International Workshop on the CCN Family of Genes. Springer Netherlands, 2010. p. 141-162.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Global expression profiling reveals a role for CTGF/CCN2 in lactogenic differentiation of mouse mammary epithelial cells

AU - Wang, Weihan

AU - Jose, Cynthia

AU - Kenney, Nicholas

AU - Morrison, Bethanie

AU - Cutler, Mary Lou

PY - 2010

Y1 - 2010

N2 - Mammary epithelial cells undergo a series of developmental changes during pregnancy and lactation including proliferation, differentiation, secretion and apoptosis. HC11 mouse mammary epithelial cells, which are capable of lactogen-induced differentiation in cell culture, were used to follow the changes in gene expression during this process. The expression profiles of over 20,000 genes were compared in HC11 cells undergoing lactogenic differentiation to non-differentiated cells using DNA microarray analysis. Greater than two fold changes were detected in 998 genes in the differentiated cells versus growth controls. Several genes, including CTGF/CCN2, exhibited greater than five-fold increase. Validation of the gene expression changes verified the involvement of numerous genes and pathways in the differentiation of mouse mammary epithelial cells and identified genetic pathways associated with specific transcriptional regulation. Further analysis revealed EGF-dependent regulation of a specific subset of genes including important cell cycle regulators. The expression of a subset of genes regulated by lactogenic differentiation in HC11 cells, including CTGF/CCN2 and osteopontin, was examined in mouse mammary glands revealing in vivo changes in expression during pregnancy and lactation. In addition, elevation or depletion of CTGF/CCN2 in HC11 cells had a significant effect on the degree of lactogenic differentiation observed in the cells. The studies confirm the value of expression profiling in defining transcriptional controls associated with differentiation of mammary epithelial cells and revealed novel regulators of the process, including CTGF/CCN2.

AB - Mammary epithelial cells undergo a series of developmental changes during pregnancy and lactation including proliferation, differentiation, secretion and apoptosis. HC11 mouse mammary epithelial cells, which are capable of lactogen-induced differentiation in cell culture, were used to follow the changes in gene expression during this process. The expression profiles of over 20,000 genes were compared in HC11 cells undergoing lactogenic differentiation to non-differentiated cells using DNA microarray analysis. Greater than two fold changes were detected in 998 genes in the differentiated cells versus growth controls. Several genes, including CTGF/CCN2, exhibited greater than five-fold increase. Validation of the gene expression changes verified the involvement of numerous genes and pathways in the differentiation of mouse mammary epithelial cells and identified genetic pathways associated with specific transcriptional regulation. Further analysis revealed EGF-dependent regulation of a specific subset of genes including important cell cycle regulators. The expression of a subset of genes regulated by lactogenic differentiation in HC11 cells, including CTGF/CCN2 and osteopontin, was examined in mouse mammary glands revealing in vivo changes in expression during pregnancy and lactation. In addition, elevation or depletion of CTGF/CCN2 in HC11 cells had a significant effect on the degree of lactogenic differentiation observed in the cells. The studies confirm the value of expression profiling in defining transcriptional controls associated with differentiation of mammary epithelial cells and revealed novel regulators of the process, including CTGF/CCN2.

KW - CTGF/CCN2

KW - Dexamethasone

KW - Lactogenic differentiation

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DO - 10.1007/978-90-481-3779-4_12

M3 - Chapter

AN - SCOPUS:84900075273

SN - 9789048137787

SP - 141

EP - 162

BT - CCN Proteins in Health and Disease

PB - Springer Netherlands

ER -

Global expression profiling reveals a role for CTGF/CCN2 in lactogenic differentiation of mouse mammary epithelial cells

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Keywords

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