Hapten-specific murine colony-forming B cells: In vitro response of colonies to fluoresceinated thymus independent antigens

P. S. Pillai, D. W. Scott

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The ability to clone hapten-specific B cells in agar and subsequently trigger their clonal progeny to antibody synthesis was investigated. Fluorescein (FL) specific B cells were purified on FL-gelatin dishes and cultured in semisolid agar for 6 to 7 days; individual colonies were then picked for restimulation in microculture. FL-specific B cells could be cloned as efficiently as unpurified splenic B cells. The number of colonies formed depended on the presence of sheep erythrocytes (SRBC) or E. coli lipopolysaccharide (LPS) in the cultures. An additive number of colonies were observed with SRBC + LPS compared to that of SRBC or LPS alone. The colonies obtained from SRBC-containing cultures were stimulatable at high frequency by various FL-conjugated antigens to yield anti-FL PFC. However, colonies grown with LPS as the only additive were not stimulatable by any of the antigens tested. On the other hand, addition of M∅ or SRBC as additional 'mitogens' along with LPS in the agar resulted in progeny colonies that could respond in vitro. Although M∅ did not increase the number of colonies, their presence enhanced the size and in some cases the frequency of stimulatable colonies. These data complement earlier observations in suggesting that different B cell subpopulations may grow under different cloning conditions. Moreover, the ability to stimulate the clonal progeny of single B cells to antibody synthesis should permit further definition of triggering and tolerance events at the single-cell level.

Original languageEnglish
Pages (from-to)1883-1886
Number of pages4
JournalJournal of Immunology
Issue number5
StatePublished - 1981
Externally publishedYes


Dive into the research topics of 'Hapten-specific murine colony-forming B cells: In vitro response of colonies to fluoresceinated thymus independent antigens'. Together they form a unique fingerprint.

Cite this