High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate

Daoqin Bi, Kevin J. Leary, Julie A. Weitz, Svetlana A. Cherstniakova, Michael A. Reil, Michael J. Roy, Louis R. Cantilena*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A simple and sensitive HPLC-UV assay was developed for the measurement of iothalamate (IOT) in human serum and urine. Chromatographic separation was achieved using an embedded-carbamate-group bonded RP18 column and mobile phase consisting of 50 mM monobasic sodium phosphate and methanol (90:10, v/v) without the addition of ion-pair reagents. The assay demonstrated a high analytical reliability within the IOT concentration range of 1-150 μg/ml in serum and 25-1500 μg/ml in urine. The relative standard deviations (RSDs) for intra- and inter-day analysis were less than 5.1% in all cases. This method has been used for the evaluation of glomerular filtration rate (GFR) in subjects participating in a phase I clinical trial of a novel antimalarial medicine. The average baseline GFR was 100.41 ± 19.99 ml/min/1.73 m2 in 119 healthy volunteers. The assay may also allow the simultaneous measurements of p-aminohippuric acid (PAH), N-acetyl PAH (aPAH), and IOT with some modification. PAH, IOT, aPAH, and β-hydroxyethyl-theophylline internal standard peaks appeared approximately at 2.5, 3.7, 5.9, and 11.8 min, respectively, in an isocratic run.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume856
Issue number1-2
DOIs
StatePublished - 1 Sep 2007

Keywords

  • BHET
  • GFR
  • HPLC-UV
  • Iothalamate

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