TY - JOUR
T1 - High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate
AU - Bi, Daoqin
AU - Leary, Kevin J.
AU - Weitz, Julie A.
AU - Cherstniakova, Svetlana A.
AU - Reil, Michael A.
AU - Roy, Michael J.
AU - Cantilena, Louis R.
PY - 2007/9/1
Y1 - 2007/9/1
N2 - A simple and sensitive HPLC-UV assay was developed for the measurement of iothalamate (IOT) in human serum and urine. Chromatographic separation was achieved using an embedded-carbamate-group bonded RP18 column and mobile phase consisting of 50 mM monobasic sodium phosphate and methanol (90:10, v/v) without the addition of ion-pair reagents. The assay demonstrated a high analytical reliability within the IOT concentration range of 1-150 μg/ml in serum and 25-1500 μg/ml in urine. The relative standard deviations (RSDs) for intra- and inter-day analysis were less than 5.1% in all cases. This method has been used for the evaluation of glomerular filtration rate (GFR) in subjects participating in a phase I clinical trial of a novel antimalarial medicine. The average baseline GFR was 100.41 ± 19.99 ml/min/1.73 m2 in 119 healthy volunteers. The assay may also allow the simultaneous measurements of p-aminohippuric acid (PAH), N-acetyl PAH (aPAH), and IOT with some modification. PAH, IOT, aPAH, and β-hydroxyethyl-theophylline internal standard peaks appeared approximately at 2.5, 3.7, 5.9, and 11.8 min, respectively, in an isocratic run.
AB - A simple and sensitive HPLC-UV assay was developed for the measurement of iothalamate (IOT) in human serum and urine. Chromatographic separation was achieved using an embedded-carbamate-group bonded RP18 column and mobile phase consisting of 50 mM monobasic sodium phosphate and methanol (90:10, v/v) without the addition of ion-pair reagents. The assay demonstrated a high analytical reliability within the IOT concentration range of 1-150 μg/ml in serum and 25-1500 μg/ml in urine. The relative standard deviations (RSDs) for intra- and inter-day analysis were less than 5.1% in all cases. This method has been used for the evaluation of glomerular filtration rate (GFR) in subjects participating in a phase I clinical trial of a novel antimalarial medicine. The average baseline GFR was 100.41 ± 19.99 ml/min/1.73 m2 in 119 healthy volunteers. The assay may also allow the simultaneous measurements of p-aminohippuric acid (PAH), N-acetyl PAH (aPAH), and IOT with some modification. PAH, IOT, aPAH, and β-hydroxyethyl-theophylline internal standard peaks appeared approximately at 2.5, 3.7, 5.9, and 11.8 min, respectively, in an isocratic run.
KW - BHET
KW - GFR
KW - HPLC-UV
KW - Iothalamate
UR - http://www.scopus.com/inward/record.url?scp=34548119817&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2007.05.045
DO - 10.1016/j.jchromb.2007.05.045
M3 - Article
C2 - 17599846
AN - SCOPUS:34548119817
SN - 1570-0232
VL - 856
SP - 95
EP - 99
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1-2
ER -