High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate

Daoqin Bi; Kevin J. Leary; Julie A. Weitz; Svetlana A. Cherstniakova; Michael A. Reil; Michael J. Roy; Louis R. Cantilena

doi:10.1016/j.jchromb.2007.05.045

High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate

Daoqin Bi, Kevin J. Leary, Julie A. Weitz, Svetlana A. Cherstniakova, Michael A. Reil, Michael J. Roy, Louis R. Cantilena^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A simple and sensitive HPLC-UV assay was developed for the measurement of iothalamate (IOT) in human serum and urine. Chromatographic separation was achieved using an embedded-carbamate-group bonded RP18 column and mobile phase consisting of 50 mM monobasic sodium phosphate and methanol (90:10, v/v) without the addition of ion-pair reagents. The assay demonstrated a high analytical reliability within the IOT concentration range of 1-150 μg/ml in serum and 25-1500 μg/ml in urine. The relative standard deviations (RSDs) for intra- and inter-day analysis were less than 5.1% in all cases. This method has been used for the evaluation of glomerular filtration rate (GFR) in subjects participating in a phase I clinical trial of a novel antimalarial medicine. The average baseline GFR was 100.41 ± 19.99 ml/min/1.73 m² in 119 healthy volunteers. The assay may also allow the simultaneous measurements of p-aminohippuric acid (PAH), N-acetyl PAH (aPAH), and IOT with some modification. PAH, IOT, aPAH, and β-hydroxyethyl-theophylline internal standard peaks appeared approximately at 2.5, 3.7, 5.9, and 11.8 min, respectively, in an isocratic run.

Original language	English
Pages (from-to)	95-99
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	856
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2007.05.045
State	Published - 1 Sep 2007
Externally published	Yes

Keywords

BHET
GFR
HPLC-UV
Iothalamate

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10.1016/j.jchromb.2007.05.045

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Bi, D., Leary, K. J., Weitz, J. A., Cherstniakova, S. A., Reil, M. A., Roy, M. J., & Cantilena, L. R. (2007). High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 856(1-2), 95-99. https://doi.org/10.1016/j.jchromb.2007.05.045

@article{4403d5a9e2114dab93586d90b51d2c98,

title = "High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate",

abstract = "A simple and sensitive HPLC-UV assay was developed for the measurement of iothalamate (IOT) in human serum and urine. Chromatographic separation was achieved using an embedded-carbamate-group bonded RP18 column and mobile phase consisting of 50 mM monobasic sodium phosphate and methanol (90:10, v/v) without the addition of ion-pair reagents. The assay demonstrated a high analytical reliability within the IOT concentration range of 1-150 μg/ml in serum and 25-1500 μg/ml in urine. The relative standard deviations (RSDs) for intra- and inter-day analysis were less than 5.1% in all cases. This method has been used for the evaluation of glomerular filtration rate (GFR) in subjects participating in a phase I clinical trial of a novel antimalarial medicine. The average baseline GFR was 100.41 ± 19.99 ml/min/1.73 m2 in 119 healthy volunteers. The assay may also allow the simultaneous measurements of p-aminohippuric acid (PAH), N-acetyl PAH (aPAH), and IOT with some modification. PAH, IOT, aPAH, and β-hydroxyethyl-theophylline internal standard peaks appeared approximately at 2.5, 3.7, 5.9, and 11.8 min, respectively, in an isocratic run.",

keywords = "BHET, GFR, HPLC-UV, Iothalamate",

author = "Daoqin Bi and Leary, {Kevin J.} and Weitz, {Julie A.} and Cherstniakova, {Svetlana A.} and Reil, {Michael A.} and Roy, {Michael J.} and Cantilena, {Louis R.}",

year = "2007",

month = sep,

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doi = "10.1016/j.jchromb.2007.05.045",

language = "English",

volume = "856",

pages = "95--99",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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number = "1-2",

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Bi, D, Leary, KJ, Weitz, JA, Cherstniakova, SA, Reil, MA, Roy, MJ & Cantilena, LR 2007, 'High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 856, no. 1-2, pp. 95-99. https://doi.org/10.1016/j.jchromb.2007.05.045

High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate. / Bi, Daoqin; Leary, Kevin J.; Weitz, Julie A. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 856, No. 1-2, 01.09.2007, p. 95-99.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate

AU - Bi, Daoqin

AU - Leary, Kevin J.

AU - Weitz, Julie A.

AU - Cherstniakova, Svetlana A.

AU - Reil, Michael A.

AU - Roy, Michael J.

AU - Cantilena, Louis R.

PY - 2007/9/1

Y1 - 2007/9/1

N2 - A simple and sensitive HPLC-UV assay was developed for the measurement of iothalamate (IOT) in human serum and urine. Chromatographic separation was achieved using an embedded-carbamate-group bonded RP18 column and mobile phase consisting of 50 mM monobasic sodium phosphate and methanol (90:10, v/v) without the addition of ion-pair reagents. The assay demonstrated a high analytical reliability within the IOT concentration range of 1-150 μg/ml in serum and 25-1500 μg/ml in urine. The relative standard deviations (RSDs) for intra- and inter-day analysis were less than 5.1% in all cases. This method has been used for the evaluation of glomerular filtration rate (GFR) in subjects participating in a phase I clinical trial of a novel antimalarial medicine. The average baseline GFR was 100.41 ± 19.99 ml/min/1.73 m2 in 119 healthy volunteers. The assay may also allow the simultaneous measurements of p-aminohippuric acid (PAH), N-acetyl PAH (aPAH), and IOT with some modification. PAH, IOT, aPAH, and β-hydroxyethyl-theophylline internal standard peaks appeared approximately at 2.5, 3.7, 5.9, and 11.8 min, respectively, in an isocratic run.

AB - A simple and sensitive HPLC-UV assay was developed for the measurement of iothalamate (IOT) in human serum and urine. Chromatographic separation was achieved using an embedded-carbamate-group bonded RP18 column and mobile phase consisting of 50 mM monobasic sodium phosphate and methanol (90:10, v/v) without the addition of ion-pair reagents. The assay demonstrated a high analytical reliability within the IOT concentration range of 1-150 μg/ml in serum and 25-1500 μg/ml in urine. The relative standard deviations (RSDs) for intra- and inter-day analysis were less than 5.1% in all cases. This method has been used for the evaluation of glomerular filtration rate (GFR) in subjects participating in a phase I clinical trial of a novel antimalarial medicine. The average baseline GFR was 100.41 ± 19.99 ml/min/1.73 m2 in 119 healthy volunteers. The assay may also allow the simultaneous measurements of p-aminohippuric acid (PAH), N-acetyl PAH (aPAH), and IOT with some modification. PAH, IOT, aPAH, and β-hydroxyethyl-theophylline internal standard peaks appeared approximately at 2.5, 3.7, 5.9, and 11.8 min, respectively, in an isocratic run.

KW - BHET

KW - GFR

KW - HPLC-UV

KW - Iothalamate

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U2 - 10.1016/j.jchromb.2007.05.045

DO - 10.1016/j.jchromb.2007.05.045

M3 - Article

C2 - 17599846

AN - SCOPUS:34548119817

SN - 1570-0232

VL - 856

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EP - 99

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

High performance liquid chromatographic measurement of iothalamate in human serum and urine for evaluation of glomerular filtration rate

Abstract

Keywords

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