High-throughput automated protein expression analysis of human breast cancer HER-2 cell lines using 2D-DIGE MALDI-MS

S. Russell; A. G. Sullivan; C. Shriver; H. Brzeski; R. I. Somiari

High-throughput automated protein expression analysis of human breast cancer HER-2 cell lines using 2D-DIGE MALDI-MS

S. Russell^*, A. G. Sullivan, C. Shriver, H. Brzeski, R. I. Somiari

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

The use of 2D-DIGE MALDI-MS technology for high-throughput automated protein expression analysis of human breast cancer HER-2 lines was discussed. For the study two cell lines were obtained from ATCC and analyzed by two-dimensional differential in gel electrophoresis. The results from the global analysis of proteins in breast cell lines by 2D-DIGE indicate that up to 3.1% of proteins may be differentially expressed between HER-2 (+) and HER-2 (-) cells. It was suggested that out of the proteins differentially expressed, 13 appear to be co-expressed with HER-2, and 9 downregulated as a response to HER-2 overexpression.

Original language	English
Pages	223-224
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

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@conference{bf3f0ab7eefb44708993a69674e198e4,

title = "High-throughput automated protein expression analysis of human breast cancer HER-2 cell lines using 2D-DIGE MALDI-MS",

abstract = "The use of 2D-DIGE MALDI-MS technology for high-throughput automated protein expression analysis of human breast cancer HER-2 lines was discussed. For the study two cell lines were obtained from ATCC and analyzed by two-dimensional differential in gel electrophoresis. The results from the global analysis of proteins in breast cell lines by 2D-DIGE indicate that up to 3.1% of proteins may be differentially expressed between HER-2 (+) and HER-2 (-) cells. It was suggested that out of the proteins differentially expressed, 13 appear to be co-expressed with HER-2, and 9 downregulated as a response to HER-2 overexpression.",

author = "S. Russell and Sullivan, {A. G.} and C. Shriver and H. Brzeski and Somiari, {R. I.}",

year = "2002",

language = "English",

pages = "223--224",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

High-throughput automated protein expression analysis of human breast cancer HER-2 cell lines using 2D-DIGE MALDI-MS. / Russell, S.; Sullivan, A. G.; Shriver, C. et al.
2002. 223-224 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - High-throughput automated protein expression analysis of human breast cancer HER-2 cell lines using 2D-DIGE MALDI-MS

AU - Russell, S.

AU - Sullivan, A. G.

AU - Shriver, C.

AU - Brzeski, H.

AU - Somiari, R. I.

PY - 2002

Y1 - 2002

N2 - The use of 2D-DIGE MALDI-MS technology for high-throughput automated protein expression analysis of human breast cancer HER-2 lines was discussed. For the study two cell lines were obtained from ATCC and analyzed by two-dimensional differential in gel electrophoresis. The results from the global analysis of proteins in breast cell lines by 2D-DIGE indicate that up to 3.1% of proteins may be differentially expressed between HER-2 (+) and HER-2 (-) cells. It was suggested that out of the proteins differentially expressed, 13 appear to be co-expressed with HER-2, and 9 downregulated as a response to HER-2 overexpression.

AB - The use of 2D-DIGE MALDI-MS technology for high-throughput automated protein expression analysis of human breast cancer HER-2 lines was discussed. For the study two cell lines were obtained from ATCC and analyzed by two-dimensional differential in gel electrophoresis. The results from the global analysis of proteins in breast cell lines by 2D-DIGE indicate that up to 3.1% of proteins may be differentially expressed between HER-2 (+) and HER-2 (-) cells. It was suggested that out of the proteins differentially expressed, 13 appear to be co-expressed with HER-2, and 9 downregulated as a response to HER-2 overexpression.

UR - http://www.scopus.com/inward/record.url?scp=2442698050&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442698050

SP - 223

EP - 224

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

High-throughput automated protein expression analysis of human breast cancer HER-2 cell lines using 2D-DIGE MALDI-MS

Abstract

Conference

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