High-throughput ballistic injection nanorheology to measure cell mechanics

Pei Hsun Wu; Christopher M. Hale; Wei Chiang Chen; Jerry S.H. Lee; Yiider Tseng; Denis Wirtz

doi:10.1038/nprot.2011.436

High-throughput ballistic injection nanorheology to measure cell mechanics

Pei Hsun Wu, Christopher M. Hale, Wei Chiang Chen, Jerry S.H. Lee, Yiider Tseng^*, Denis Wirtz

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

High-throughput ballistic injection nanorheology is a method for the quantitative study of cell mechanics. Ccell mechanics are measured by ballistic injection of submicron particles into the cytoplasm of living cells and tracking the spontaneous displacement of the particles at high spatial resolution. Tthe trajectories of the cytoplasm-embedded particles are transformed into mean-squared displacements, which are subsequently transformed into frequency-dependent viscoelastic moduli and time-dependent creep compliance of the cytoplasm. Tthis method allows for the study of a wide range of cellular conditions, including cells inside a 3D matrix, cell subjected to shear flows and biochemical stimuli, and cells in a live animal. Ballistic injection lasts < 1 min and is followed by overnight incubation. Multiple particle tracking for one cell lasts < 1 min. Forty cells can be examined in < 1 h.

Original language	English
Pages (from-to)	155-170
Number of pages	16
Journal	Nature Protocols
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/nprot.2011.436
State	Published - Jan 2012
Externally published	Yes

Access to Document

10.1038/nprot.2011.436

Cite this

@article{b64bf7da5b3f4277a523426446ca7327,

title = "High-throughput ballistic injection nanorheology to measure cell mechanics",

abstract = "High-throughput ballistic injection nanorheology is a method for the quantitative study of cell mechanics. Ccell mechanics are measured by ballistic injection of submicron particles into the cytoplasm of living cells and tracking the spontaneous displacement of the particles at high spatial resolution. Tthe trajectories of the cytoplasm-embedded particles are transformed into mean-squared displacements, which are subsequently transformed into frequency-dependent viscoelastic moduli and time-dependent creep compliance of the cytoplasm. Tthis method allows for the study of a wide range of cellular conditions, including cells inside a 3D matrix, cell subjected to shear flows and biochemical stimuli, and cells in a live animal. Ballistic injection lasts < 1 min and is followed by overnight incubation. Multiple particle tracking for one cell lasts < 1 min. Forty cells can be examined in < 1 h.",

author = "Wu, {Pei Hsun} and Hale, {Christopher M.} and Chen, {Wei Chiang} and Lee, {Jerry S.H.} and Yiider Tseng and Denis Wirtz",

year = "2012",

month = jan,

doi = "10.1038/nprot.2011.436",

language = "English",

volume = "7",

pages = "155--170",

journal = "Nature Protocols",

issn = "1754-2189",

number = "1",

}

TY - JOUR

T1 - High-throughput ballistic injection nanorheology to measure cell mechanics

AU - Wu, Pei Hsun

AU - Hale, Christopher M.

AU - Chen, Wei Chiang

AU - Lee, Jerry S.H.

AU - Tseng, Yiider

AU - Wirtz, Denis

PY - 2012/1

Y1 - 2012/1

N2 - High-throughput ballistic injection nanorheology is a method for the quantitative study of cell mechanics. Ccell mechanics are measured by ballistic injection of submicron particles into the cytoplasm of living cells and tracking the spontaneous displacement of the particles at high spatial resolution. Tthe trajectories of the cytoplasm-embedded particles are transformed into mean-squared displacements, which are subsequently transformed into frequency-dependent viscoelastic moduli and time-dependent creep compliance of the cytoplasm. Tthis method allows for the study of a wide range of cellular conditions, including cells inside a 3D matrix, cell subjected to shear flows and biochemical stimuli, and cells in a live animal. Ballistic injection lasts < 1 min and is followed by overnight incubation. Multiple particle tracking for one cell lasts < 1 min. Forty cells can be examined in < 1 h.

AB - High-throughput ballistic injection nanorheology is a method for the quantitative study of cell mechanics. Ccell mechanics are measured by ballistic injection of submicron particles into the cytoplasm of living cells and tracking the spontaneous displacement of the particles at high spatial resolution. Tthe trajectories of the cytoplasm-embedded particles are transformed into mean-squared displacements, which are subsequently transformed into frequency-dependent viscoelastic moduli and time-dependent creep compliance of the cytoplasm. Tthis method allows for the study of a wide range of cellular conditions, including cells inside a 3D matrix, cell subjected to shear flows and biochemical stimuli, and cells in a live animal. Ballistic injection lasts < 1 min and is followed by overnight incubation. Multiple particle tracking for one cell lasts < 1 min. Forty cells can be examined in < 1 h.

UR - http://www.scopus.com/inward/record.url?scp=84861113455&partnerID=8YFLogxK

U2 - 10.1038/nprot.2011.436

DO - 10.1038/nprot.2011.436

M3 - Article

C2 - 22222790

AN - SCOPUS:84861113455

SN - 1754-2189

VL - 7

SP - 155

EP - 170

JO - Nature Protocols

JF - Nature Protocols

IS - 1

ER -

High-throughput ballistic injection nanorheology to measure cell mechanics

Abstract

Access to Document

Fingerprint

Cite this