High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class i ligands using real-time PCR

R. N. Koehler; A. M. Walsh; N. Moqueet; J. R. Currier; M. A. Eller; L. A. Eller; F. Wabwire-Mangen; N. L. Michael; M. L. Robb; F. E. McCutchan; G. H. Kijak

doi:10.1111/j.1399-0039.2009.01265.x

High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class i ligands using real-time PCR

R. N. Koehler, A. M. Walsh, N. Moqueet, J. R. Currier, M. A. Eller, L. A. Eller, F. Wabwire-Mangen, N. L. Michael, M. L. Robb, F. E. McCutchan, G. H. Kijak

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Killer immunoglobulin-like receptors (KIRs) expressed on natural killer cells are critical components of innate immunity. Interactions between KIRs and their human leukocyte antigen (HLA) ligands have been shown to influence autoimmune and infectious disease course in defined populations. However, the low throughput and high cost of current methods impede confirmation of the universality of these findings. To support large epidemiology surveys, we developed a high-throughput real-time polymerase chain reaction-based assay to identify carriers of KIR3DL1, KIR3DS1, KIR2DL2, and KIR2DL3 and their HLA ligands. The platform performed with 100% sensitivity and specificity in detection of carrier and non-carrier on reference samples. The application of this platform will further clarify the nature and impact of the KIR-HLA epistatic interaction on disease course in large global population-based studies.

Original language	English
Pages (from-to)	73-80
Number of pages	8
Journal	Tissue Antigens
Volume	74
Issue number	1
DOIs	https://doi.org/10.1111/j.1399-0039.2009.01265.x
State	Published - Jul 2009
Externally published	Yes

Keywords

Human leukocyte antigen
Innate immunity
Killer immunoglobulin-like receptor
Real-time polymerase chain reaction
TaqMan

Access to Document

10.1111/j.1399-0039.2009.01265.x

Cite this

@article{8883b55840b44b78bb4f2d35bb5eab50,

title = "High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class i ligands using real-time PCR",

abstract = "Killer immunoglobulin-like receptors (KIRs) expressed on natural killer cells are critical components of innate immunity. Interactions between KIRs and their human leukocyte antigen (HLA) ligands have been shown to influence autoimmune and infectious disease course in defined populations. However, the low throughput and high cost of current methods impede confirmation of the universality of these findings. To support large epidemiology surveys, we developed a high-throughput real-time polymerase chain reaction-based assay to identify carriers of KIR3DL1, KIR3DS1, KIR2DL2, and KIR2DL3 and their HLA ligands. The platform performed with 100% sensitivity and specificity in detection of carrier and non-carrier on reference samples. The application of this platform will further clarify the nature and impact of the KIR-HLA epistatic interaction on disease course in large global population-based studies.",

keywords = "Human leukocyte antigen, Innate immunity, Killer immunoglobulin-like receptor, Real-time polymerase chain reaction, TaqMan",

author = "Koehler, {R. N.} and Walsh, {A. M.} and N. Moqueet and Currier, {J. R.} and Eller, {M. A.} and Eller, {L. A.} and F. Wabwire-Mangen and Michael, {N. L.} and Robb, {M. L.} and McCutchan, {F. E.} and Kijak, {G. H.}",

year = "2009",

month = jul,

doi = "10.1111/j.1399-0039.2009.01265.x",

language = "English",

volume = "74",

pages = "73--80",

journal = "Tissue Antigens",

issn = "0001-2815",

number = "1",

}

TY - JOUR

T1 - High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class i ligands using real-time PCR

AU - Koehler, R. N.

AU - Walsh, A. M.

AU - Moqueet, N.

AU - Currier, J. R.

AU - Eller, M. A.

AU - Eller, L. A.

AU - Wabwire-Mangen, F.

AU - Michael, N. L.

AU - Robb, M. L.

AU - McCutchan, F. E.

AU - Kijak, G. H.

PY - 2009/7

Y1 - 2009/7

N2 - Killer immunoglobulin-like receptors (KIRs) expressed on natural killer cells are critical components of innate immunity. Interactions between KIRs and their human leukocyte antigen (HLA) ligands have been shown to influence autoimmune and infectious disease course in defined populations. However, the low throughput and high cost of current methods impede confirmation of the universality of these findings. To support large epidemiology surveys, we developed a high-throughput real-time polymerase chain reaction-based assay to identify carriers of KIR3DL1, KIR3DS1, KIR2DL2, and KIR2DL3 and their HLA ligands. The platform performed with 100% sensitivity and specificity in detection of carrier and non-carrier on reference samples. The application of this platform will further clarify the nature and impact of the KIR-HLA epistatic interaction on disease course in large global population-based studies.

AB - Killer immunoglobulin-like receptors (KIRs) expressed on natural killer cells are critical components of innate immunity. Interactions between KIRs and their human leukocyte antigen (HLA) ligands have been shown to influence autoimmune and infectious disease course in defined populations. However, the low throughput and high cost of current methods impede confirmation of the universality of these findings. To support large epidemiology surveys, we developed a high-throughput real-time polymerase chain reaction-based assay to identify carriers of KIR3DL1, KIR3DS1, KIR2DL2, and KIR2DL3 and their HLA ligands. The platform performed with 100% sensitivity and specificity in detection of carrier and non-carrier on reference samples. The application of this platform will further clarify the nature and impact of the KIR-HLA epistatic interaction on disease course in large global population-based studies.

KW - Human leukocyte antigen

KW - Innate immunity

KW - Killer immunoglobulin-like receptor

KW - Real-time polymerase chain reaction

KW - TaqMan

UR - http://www.scopus.com/inward/record.url?scp=66949161526&partnerID=8YFLogxK

U2 - 10.1111/j.1399-0039.2009.01265.x

DO - 10.1111/j.1399-0039.2009.01265.x

M3 - Article

C2 - 19522772

AN - SCOPUS:66949161526

SN - 0001-2815

VL - 74

SP - 73

EP - 80

JO - Tissue Antigens

JF - Tissue Antigens

IS - 1

ER -

High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class i ligands using real-time PCR

Abstract

Keywords

Access to Document

Fingerprint

Cite this