TY - JOUR
T1 - Host CD3+ T-cells can significantly modulate phage treatment effects on bacterial bioburden in mouse models
AU - Li, Renhua
AU - Rouse, Michael
AU - Pace, Brendon T
AU - Grey, Scott F
AU - Mclaughlin, Kimberly
AU - Schobel, Seth A
AU - Simons, Mark P
N1 - Copyright © 2023 Li, Rouse, Pace, Grey, Mclaughlin, Schobel and Simons.
PY - 2023
Y1 - 2023
N2 - Wound healing is a complex system including such key players as host, microbe, and treatments. However, little is known about their dynamic interactions. Here we explored the interplay between: (1) bacterial bioburden and host immune responses, (2) bacterial bioburden and wound size, and (3) treatments and wound size, using murine models and various treatment modalities: Phosphate buffer saline (PBS or vehicle, negative control), doxycycline, and two doses of A. baumannii phage mixtures. We uncovered that the interplay between bacterial bioburden and host immune system may be bidirectional, and that there is an interaction between host CD3+ T-cells and phage dosage, which significantly impacts bacterial bioburden. Furthermore, the bacterial bioburden and wound size association is significantly modulated by the host CD3+ T-cells. When the host CD3+ T-cells (x on log10 scale) are in the appropriate range (1.35 < x < = 1.5), we observed a strong association between colony forming units (CFU) and wound size, indicating a hallmark of wound healing. On the basis of the findings and our previous work, we proposed an integrated parallel systems biology model.
AB - Wound healing is a complex system including such key players as host, microbe, and treatments. However, little is known about their dynamic interactions. Here we explored the interplay between: (1) bacterial bioburden and host immune responses, (2) bacterial bioburden and wound size, and (3) treatments and wound size, using murine models and various treatment modalities: Phosphate buffer saline (PBS or vehicle, negative control), doxycycline, and two doses of A. baumannii phage mixtures. We uncovered that the interplay between bacterial bioburden and host immune system may be bidirectional, and that there is an interaction between host CD3+ T-cells and phage dosage, which significantly impacts bacterial bioburden. Furthermore, the bacterial bioburden and wound size association is significantly modulated by the host CD3+ T-cells. When the host CD3+ T-cells (x on log10 scale) are in the appropriate range (1.35 < x < = 1.5), we observed a strong association between colony forming units (CFU) and wound size, indicating a hallmark of wound healing. On the basis of the findings and our previous work, we proposed an integrated parallel systems biology model.
U2 - 10.3389/fmicb.2023.1240176
DO - 10.3389/fmicb.2023.1240176
M3 - Article
C2 - 37766890
SN - 1664-302X
VL - 14
SP - 1240176
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
ER -