The resistance of two biomaterials, one synthetic and one biologic in origin, to deliberate bacterial infection was compared in a dog model of orthopedic soft tissue reconstruction. Twenty-four adult female dogs were randomly divided into two equal groups and a 2.0-cm-round full-thickness defect was created on the lateral surface of the stifle joint, leaving only the synovium and skin intact. The defect was surgically repaired with either Dacron™ mesh or a porcine derived extracellular matrix (ECM) scaffold material. The repair site was inoculated with 1 × 108 Staphylococcus aureus at the time of surgery and the dogs were survived for 28 days. Results showed a chronic pyogranulomatous inflammatory response at the Dacron™ implant sites versus a constructive tissue-remodeling response without residual inflammation at the ECM implant site. Three dogs in the group receiving the Dacron™ mesh were treated with Keflex™ (500 mg bid x 7 days) for signs of septicemia. A quantitative bacterial count of the implant sites at the time of sacrifice showed 6.52 × 105 ± 1.2 × 106 and 6.5 × 102 ± 1.8 × 103 bacteria per gram of tissue for the Dacron™ and ECM scaffold sites, respectively (P < .03). The ECM implant material was more resistant than the synthetic implant material to persistent infection following deliberate bacterial contamination and the ECM scaffold supported constructive tissue remodeling.
|Number of pages||7|
|Journal||Journal of Biomedical Materials Research - Part B Applied Biomaterials|
|State||Published - 15 Oct 2003|
- ECM-extracellular matrix
- Infection resistance
- Orthopedic surgery