Host Protection against Deliberate Bacterial Contamination of an Extracellular Matrix Bioscaffold versus Dacron™ Mesh in a Dog Model of Orthopedic Soft Tissue Repair

Stephen F. Badylak*, Ching Ching Wu, Melissa Bible, Edward McPherson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The resistance of two biomaterials, one synthetic and one biologic in origin, to deliberate bacterial infection was compared in a dog model of orthopedic soft tissue reconstruction. Twenty-four adult female dogs were randomly divided into two equal groups and a 2.0-cm-round full-thickness defect was created on the lateral surface of the stifle joint, leaving only the synovium and skin intact. The defect was surgically repaired with either Dacron™ mesh or a porcine derived extracellular matrix (ECM) scaffold material. The repair site was inoculated with 1 × 108 Staphylococcus aureus at the time of surgery and the dogs were survived for 28 days. Results showed a chronic pyogranulomatous inflammatory response at the Dacron™ implant sites versus a constructive tissue-remodeling response without residual inflammation at the ECM implant site. Three dogs in the group receiving the Dacron™ mesh were treated with Keflex™ (500 mg bid x 7 days) for signs of septicemia. A quantitative bacterial count of the implant sites at the time of sacrifice showed 6.52 × 105 ± 1.2 × 106 and 6.5 × 102 ± 1.8 × 103 bacteria per gram of tissue for the Dacron™ and ECM scaffold sites, respectively (P < .03). The ECM implant material was more resistant than the synthetic implant material to persistent infection following deliberate bacterial contamination and the ECM scaffold supported constructive tissue remodeling.

Original languageEnglish
Pages (from-to)648-654
Number of pages7
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume67
Issue number1
DOIs
StatePublished - 15 Oct 2003
Externally publishedYes

Keywords

  • Biomaterial
  • ECM-extracellular matrix
  • Infection resistance
  • Orthopedic surgery
  • Scaffold

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