HSPD1, HSPB1 and VDAC1 are over-expressed in invasive ductal carcinoma of the breast

Mutiu A. Alabi; Olugbenga O. Adebawo; Oluwole A. Daini; Stella B. Somiari; Richard I. Somiari

doi:10.3923/ijcr.2016.82.91

HSPD1, HSPB1 and VDAC1 are over-expressed in invasive ductal carcinoma of the breast

Mutiu A. Alabi^*, Olugbenga O. Adebawo, Oluwole A. Daini, Stella B. Somiari, Richard I. Somiari

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Background and Objectives: The initiating steps and precise pathway of breast tumorigenesis are poorly understood and it is unclear if Ductal Carcinoma In Situ (DCIS) progresses to invasive ductal carcinoma (IDCA) of the breast. This study was undertaken to identify proteins that are differentially expressed between IDCA and DCIS and that may predict the invasive potential of breast tumors. Methodology: It is utilized that the two-dimensional difference in gel electrophoresis technology (2D-DIGE) and tandem mass spectrometry (LC-MS/MS) to perform proteomic analysis of IDCA (MCF-7 and BT-474) and DCIS (HCC-1500 and HCC-B8) cell lines. Results: Identified 10 proteins that were differentially expressed between IDCA and DCIS (≥2-fold difference; p≤0.05) and classified the proteins according to their Gene Ontology (GO). Out of these proteins, 60 kDa mitochondrial heat shock protein (HSPD1), Heat Shock Protein Beta 1 (HSPB1) and the voltage-dependent anion-selective channel protein 1 (VDAC1) are over expressed in IDCA compared to DCIS. Conclusion: The functional role of the differentially expressed proteins suggests that they may serve as biomarkers for identification of tumors with invasive potential.

Original language	English
Pages (from-to)	82-91
Number of pages	10
Journal	International Journal of Cancer Research
Volume	12
Issue number	2
DOIs	https://doi.org/10.3923/ijcr.2016.82.91
State	Published - 15 Mar 2016
Externally published	Yes

Keywords

Breast cancer
Heat shock protein
Mass spectrometry
Two-dimensional difference gel electrophoresis

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title = "HSPD1, HSPB1 and VDAC1 are over-expressed in invasive ductal carcinoma of the breast",

abstract = "Background and Objectives: The initiating steps and precise pathway of breast tumorigenesis are poorly understood and it is unclear if Ductal Carcinoma In Situ (DCIS) progresses to invasive ductal carcinoma (IDCA) of the breast. This study was undertaken to identify proteins that are differentially expressed between IDCA and DCIS and that may predict the invasive potential of breast tumors. Methodology: It is utilized that the two-dimensional difference in gel electrophoresis technology (2D-DIGE) and tandem mass spectrometry (LC-MS/MS) to perform proteomic analysis of IDCA (MCF-7 and BT-474) and DCIS (HCC-1500 and HCC-B8) cell lines. Results: Identified 10 proteins that were differentially expressed between IDCA and DCIS (≥2-fold difference; p≤0.05) and classified the proteins according to their Gene Ontology (GO). Out of these proteins, 60 kDa mitochondrial heat shock protein (HSPD1), Heat Shock Protein Beta 1 (HSPB1) and the voltage-dependent anion-selective channel protein 1 (VDAC1) are over expressed in IDCA compared to DCIS. Conclusion: The functional role of the differentially expressed proteins suggests that they may serve as biomarkers for identification of tumors with invasive potential.",

keywords = "Breast cancer, Heat shock protein, Mass spectrometry, Two-dimensional difference gel electrophoresis",

author = "Alabi, {Mutiu A.} and Adebawo, {Olugbenga O.} and Daini, {Oluwole A.} and Somiari, {Stella B.} and Somiari, {Richard I.}",

note = "Publisher Copyright: {\textcopyright} 2016 Mutiu A. Alabi et al.",

year = "2016",

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doi = "10.3923/ijcr.2016.82.91",

language = "English",

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T1 - HSPD1, HSPB1 and VDAC1 are over-expressed in invasive ductal carcinoma of the breast

AU - Alabi, Mutiu A.

AU - Adebawo, Olugbenga O.

AU - Daini, Oluwole A.

AU - Somiari, Stella B.

AU - Somiari, Richard I.

PY - 2016/3/15

Y1 - 2016/3/15

N2 - Background and Objectives: The initiating steps and precise pathway of breast tumorigenesis are poorly understood and it is unclear if Ductal Carcinoma In Situ (DCIS) progresses to invasive ductal carcinoma (IDCA) of the breast. This study was undertaken to identify proteins that are differentially expressed between IDCA and DCIS and that may predict the invasive potential of breast tumors. Methodology: It is utilized that the two-dimensional difference in gel electrophoresis technology (2D-DIGE) and tandem mass spectrometry (LC-MS/MS) to perform proteomic analysis of IDCA (MCF-7 and BT-474) and DCIS (HCC-1500 and HCC-B8) cell lines. Results: Identified 10 proteins that were differentially expressed between IDCA and DCIS (≥2-fold difference; p≤0.05) and classified the proteins according to their Gene Ontology (GO). Out of these proteins, 60 kDa mitochondrial heat shock protein (HSPD1), Heat Shock Protein Beta 1 (HSPB1) and the voltage-dependent anion-selective channel protein 1 (VDAC1) are over expressed in IDCA compared to DCIS. Conclusion: The functional role of the differentially expressed proteins suggests that they may serve as biomarkers for identification of tumors with invasive potential.

AB - Background and Objectives: The initiating steps and precise pathway of breast tumorigenesis are poorly understood and it is unclear if Ductal Carcinoma In Situ (DCIS) progresses to invasive ductal carcinoma (IDCA) of the breast. This study was undertaken to identify proteins that are differentially expressed between IDCA and DCIS and that may predict the invasive potential of breast tumors. Methodology: It is utilized that the two-dimensional difference in gel electrophoresis technology (2D-DIGE) and tandem mass spectrometry (LC-MS/MS) to perform proteomic analysis of IDCA (MCF-7 and BT-474) and DCIS (HCC-1500 and HCC-B8) cell lines. Results: Identified 10 proteins that were differentially expressed between IDCA and DCIS (≥2-fold difference; p≤0.05) and classified the proteins according to their Gene Ontology (GO). Out of these proteins, 60 kDa mitochondrial heat shock protein (HSPD1), Heat Shock Protein Beta 1 (HSPB1) and the voltage-dependent anion-selective channel protein 1 (VDAC1) are over expressed in IDCA compared to DCIS. Conclusion: The functional role of the differentially expressed proteins suggests that they may serve as biomarkers for identification of tumors with invasive potential.

KW - Breast cancer

KW - Heat shock protein

KW - Mass spectrometry

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HSPD1, HSPB1 and VDAC1 are over-expressed in invasive ductal carcinoma of the breast

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Keywords

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