hUBC9 associates with MEKK1 and type I TNF-α receptor and stimulates NFκB activity

Alan Saltzman; George Searfoss; Christophe Marcireau; Maureen Stone; Rose Ressner; Robin Munro; Carol Franks; Jill D'Alonzo; Bruno Tocque; Michael Jaye; Yuri Ivashchenko

doi:10.1016/S0014-5793(98)00287-7

hUBC9 associates with MEKK1 and type I TNF-α receptor and stimulates NFκB activity

Alan Saltzman, George Searfoss, Christophe Marcireau, Maureen Stone, Rose Ressner, Robin Munro, Carol Franks, Jill D'Alonzo, Bruno Tocque, Michael Jaye, Yuri Ivashchenko^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

hUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two-hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF-α receptor, respectively. Because both of these proteins regulate NFκB activity, the role of hUBC9 in modulating NFκB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFκB as detetermined by NFκB reporter and IL-6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFκB reporter activity. Thus, hUBC9 modulates NFκB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF-α receptor.

Original language	English
Pages (from-to)	431-435
Number of pages	5
Journal	FEBS Letters
Volume	425
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00287-7
State	Published - 3 Apr 1998
Externally published	Yes

Keywords

MEKK1
Nuclear factor κB
Tumor necrosis factor-α
Ubiquitin conjugating enzyme
Yeast two-hybrid

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10.1016/S0014-5793(98)00287-7

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title = "hUBC9 associates with MEKK1 and type I TNF-α receptor and stimulates NFκB activity",

abstract = "hUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two-hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF-α receptor, respectively. Because both of these proteins regulate NFκB activity, the role of hUBC9 in modulating NFκB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFκB as detetermined by NFκB reporter and IL-6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFκB reporter activity. Thus, hUBC9 modulates NFκB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF-α receptor.",

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author = "Alan Saltzman and George Searfoss and Christophe Marcireau and Maureen Stone and Rose Ressner and Robin Munro and Carol Franks and Jill D'Alonzo and Bruno Tocque and Michael Jaye and Yuri Ivashchenko",

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T1 - hUBC9 associates with MEKK1 and type I TNF-α receptor and stimulates NFκB activity

AU - Saltzman, Alan

AU - Searfoss, George

AU - Marcireau, Christophe

AU - Stone, Maureen

AU - Ressner, Rose

AU - Munro, Robin

AU - Franks, Carol

AU - D'Alonzo, Jill

AU - Tocque, Bruno

AU - Jaye, Michael

AU - Ivashchenko, Yuri

PY - 1998/4/3

Y1 - 1998/4/3

N2 - hUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two-hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF-α receptor, respectively. Because both of these proteins regulate NFκB activity, the role of hUBC9 in modulating NFκB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFκB as detetermined by NFκB reporter and IL-6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFκB reporter activity. Thus, hUBC9 modulates NFκB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF-α receptor.

AB - hUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two-hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF-α receptor, respectively. Because both of these proteins regulate NFκB activity, the role of hUBC9 in modulating NFκB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFκB as detetermined by NFκB reporter and IL-6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFκB reporter activity. Thus, hUBC9 modulates NFκB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF-α receptor.

KW - MEKK1

KW - Nuclear factor κB

KW - Tumor necrosis factor-α

KW - Ubiquitin conjugating enzyme

KW - Yeast two-hybrid

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ER -

hUBC9 associates with MEKK1 and type I TNF-α receptor and stimulates NFκB activity

Abstract

Keywords

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