Human mesenchymal stem cells seeded on extracellular matrix-scaffold: Viability and osteogenic potential

Letizia Penolazzi, Stefania Mazzitelli, Renata Vecchiatini, Elena Torreggiani, Elisabetta Lambertini, Scott Johnson, Stephen F. Badylak, Roberta Piva*, Claudio Nastruzzi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


The development and the optimization of novel culture systems of mesenchymal osteoprogenitors are some of the most important challenges in the field of bone tissue engineering (TE). A new combination between cells and extracellular matrix (ECM)-scaffold, containing ECM has here been analyzed. As source for osteoprogenitors, mesenchymal stem cells obtained from human umbilical cord Wharton's Jelly (hWJMSCs), were used. As ECM-scaffold, a powder form of isolated and purified porcine urinary bladder matrix (pUBM), was employed. The goals of the current work were: (1) the characterization of the in vitro hWJMSCs behavior, in terms of viability, proliferation, and adhesion to ECM-scaffold; (2) the effectiveness of ECM-scaffold to induce/modulate the osteoblastic differentiation; and (3) the proposal for a possible application of cells/ECM-scaffold construct to the field of cell/TE. In this respect, the properties of the pUBM-scaffold in promoting and guiding the in vitro adhesion, proliferation, and three-dimensional colonization of hWJMSCs, without altering viability and morphological characteristics of the cells, are here described. Finally, we have also demonstrated that pUBM-scaffolds positively affect the expression of typical osteoblastic markers in hWJMSCs.

Original languageEnglish
Pages (from-to)857-866
Number of pages10
JournalJournal of Cellular Physiology
Issue number2
StatePublished - Jan 2012
Externally publishedYes


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