Hyperbaric treatment of platelets is comparable to cold storage alone over 14 days

Kristin M. Reddoch-Cardenas*, Heather F. Pidcoke, Anand K. Ramasubramanian, M. Adam Meledeo, Andrew P. Cap

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Background: Platelets stored at room temperature (22–24°C) for transfusion purposes have a shelf life of 5–7 days, or 72 h when stored refrigerated (1–6°C). The limited shelf life of platelet products severely compromises platelet inventory. We hypothesized that cold storage of platelets in 100% plasma using xenon gas under high pressure would extend shelf life to 14 days. Study Design and Methods: Double apheresis platelet units were collected and split equally between two bags. One unit was placed in a hyperbaric chamber, pressurized to 4 bars with a xenon/oxygen gas mixture, and placed in a refrigerator for 14 days (Xe). The remaining unit was aliquoted into mini-bags (10 ml) for storage at room temperature (RTP) or in cold (CSP). Samples were assayed on days 5 (RTP) or 14 (Xe and CSP) for count, metabolism, clot strength, platelet aggregation, and activation markers. Results: The platelet count in Xe samples was lower than that of RTP but significantly higher than CSP. Despite similar levels of glucose and lactate, the pH of Xe samples was significantly lower than CSP. Glycoprotein expression was better preserved by Xe storage compared to CSP, but no differences in activation were observed. Thromboelastography and aggregometry results were comparable between all groups. Discussion: Cold storage of platelets in plasma with hyperbaric xenon provides no significant improvement in platelet function over cold storage alone. The use of a hyperbaric chamber and the slow off-gassing of Xe-stored units complicate platelet storage and delivery logistics.

Original languageEnglish
Pages (from-to)S120-S125
Issue numberS3
StatePublished - May 2023
Externally publishedYes


  • hematology—platelets
  • hemostasis
  • therapeutic apheresis


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