TY - JOUR
T1 - Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice
AU - Ivanov, Yury V.
AU - Linz, Bodo
AU - Register, Karen B.
AU - Newman, Jeffrey D.
AU - Taylor, Dawn L.
AU - Boschert, Kenneth R.
AU - Le Guyon, Soazig
AU - Wilson, Emily F.
AU - Brinkac, Lauren M.
AU - Sanka, Ravi
AU - Greco, Suellen C.
AU - Klender, Paula M.
AU - Losada, Liliana
AU - Harvill, Eric T.
N1 - Publisher Copyright:
© 2016 IUMS.
PY - 2016/12
Y1 - 2016/12
N2 - Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.
AB - Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.
UR - http://www.scopus.com/inward/record.url?scp=85009969352&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001540
DO - 10.1099/ijsem.0.001540
M3 - Article
C2 - 27707434
AN - SCOPUS:85009969352
SN - 1466-5026
VL - 66
SP - 5452
EP - 5459
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 12
ER -