Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice

Yury V. Ivanov; Bodo Linz; Karen B. Register; Jeffrey D. Newman; Dawn L. Taylor; Kenneth R. Boschert; Soazig Le Guyon; Emily F. Wilson; Lauren M. Brinkac; Ravi Sanka; Suellen C. Greco; Paula M. Klender; Liliana Losada; Eric T. Harvill

doi:10.1099/ijsem.0.001540

Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice

Yury V. Ivanov^*
, Bodo Linz
, Karen B. Register
, Jeffrey D. Newman
, Dawn L. Taylor
, Kenneth R. Boschert
, Soazig Le Guyon
, Emily F. Wilson
, Lauren M. Brinkac
, Ravi Sanka
, Suellen C. Greco
, Paula M. Klender
, Liliana Losada
, Eric T. Harvill

^*Corresponding author for this work

Surgery

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35 Scopus citations

Abstract

Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03^T (=NRRL B-59942^T=NCTC 13808^T) that infect mice.

Original language	English
Pages (from-to)	5452-5459
Number of pages	8
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	66
Issue number	12
DOIs	https://doi.org/10.1099/ijsem.0.001540
State	Published - Dec 2016

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Ivanov, Y. V., Linz, B., Register, K. B., Newman, J. D., Taylor, D. L., Boschert, K. R., Le Guyon, S., Wilson, E. F., Brinkac, L. M., Sanka, R., Greco, S. C., Klender, P. M., Losada, L., & Harvill, E. T. (2016). Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice. International Journal of Systematic and Evolutionary Microbiology, 66(12), 5452-5459. https://doi.org/10.1099/ijsem.0.001540

@article{0708ea37959041bbb2b1946694254bcd,

title = "Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice",

abstract = "Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.",

author = "Ivanov, \{Yury V.\} and Bodo Linz and Register, \{Karen B.\} and Newman, \{Jeffrey D.\} and Taylor, \{Dawn L.\} and Boschert, \{Kenneth R.\} and \{Le Guyon\}, Soazig and Wilson, \{Emily F.\} and Brinkac, \{Lauren M.\} and Ravi Sanka and Greco, \{Suellen C.\} and Klender, \{Paula M.\} and Liliana Losada and Harvill, \{Eric T.\}",

note = "Publisher Copyright: {\textcopyright} 2016 IUMS.",

year = "2016",

month = dec,

doi = "10.1099/ijsem.0.001540",

language = "English",

volume = "66",

pages = "5452--5459",

journal = "International Journal of Systematic and Evolutionary Microbiology",

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Ivanov, YV, Linz, B, Register, KB, Newman, JD, Taylor, DL, Boschert, KR, Le Guyon, S, Wilson, EF, Brinkac, LM, Sanka, R, Greco, SC, Klender, PM, Losada, L & Harvill, ET 2016, 'Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice', International Journal of Systematic and Evolutionary Microbiology, vol. 66, no. 12, pp. 5452-5459. https://doi.org/10.1099/ijsem.0.001540

TY - JOUR

T1 - Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice

AU - Ivanov, Yury V.

AU - Linz, Bodo

AU - Register, Karen B.

AU - Newman, Jeffrey D.

AU - Taylor, Dawn L.

AU - Boschert, Kenneth R.

AU - Le Guyon, Soazig

AU - Wilson, Emily F.

AU - Brinkac, Lauren M.

AU - Sanka, Ravi

AU - Greco, Suellen C.

AU - Klender, Paula M.

AU - Losada, Liliana

AU - Harvill, Eric T.

PY - 2016/12

Y1 - 2016/12

N2 - Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.

AB - Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.

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DO - 10.1099/ijsem.0.001540

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AN - SCOPUS:85009969352

SN - 1466-5026

VL - 66

SP - 5452

EP - 5459

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 12

ER -

Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice

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