Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China

Wu Chun Cao; Yu Min Gao; Pan He Zhang; Xi Tan Zhang; Qing Hua Dai; J. Stephen Dumler; Li Qun Fang; Hong Yang

doi:10.1128/jcm.38.7.2778-2780.2000

Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China

Wu Chun Cao^*
, Yu Min Gao
, Pan He Zhang
, Xi Tan Zhang
, Qing Hua Dai
, J. Stephen Dumler
, Li Qun Fang
, Hong Yang

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55.2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.

Original language	English
Pages (from-to)	2778-2780
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	38
Issue number	7
DOIs	https://doi.org/10.1128/jcm.38.7.2778-2780.2000
State	Published - 2000

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10.1128/jcm.38.7.2778-2780.2000

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title = "Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China",

abstract = "A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55.2\%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7\%) of 240 adult and at least 4.2\% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.",

author = "Cao, \{Wu Chun\} and Gao, \{Yu Min\} and Zhang, \{Pan He\} and Zhang, \{Xi Tan\} and Dai, \{Qing Hua\} and Dumler, \{J. Stephen\} and Fang, \{Li Qun\} and Hong Yang",

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T1 - Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China

AU - Cao, Wu Chun

AU - Gao, Yu Min

AU - Zhang, Pan He

AU - Zhang, Xi Tan

AU - Dai, Qing Hua

AU - Dumler, J. Stephen

AU - Fang, Li Qun

AU - Yang, Hong

PY - 2000

Y1 - 2000

N2 - A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55.2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.

AB - A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55.2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.

UR - https://www.scopus.com/pages/publications/0033929306

U2 - 10.1128/jcm.38.7.2778-2780.2000

DO - 10.1128/jcm.38.7.2778-2780.2000

M3 - Article

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AN - SCOPUS:0033929306

SN - 0095-1137

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SP - 2778

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China

Abstract

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