Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E

Silvia Ratto-Kim; Mark S. De Souza; Jeffrey R. Currier; Nicos Karasavvas; John Sidney; Morgane Rolland; Anais Valencia-Micolta; Sirinan Madnote; Alessandro Sette; Sorachai Nitayaphan; Punnee Pitisuttuthum; Jaranit Kaewkungwal; Supachai Rerks-Ngarm; Robert O'Connell; Nelson Michael; Merlin L. Robb; Mary Marovich; Jerome H. Kim

doi:10.1371/journal.pone.0115582

Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E

Silvia Ratto-Kim, Mark S. De Souza^*, Jeffrey R. Currier, Nicos Karasavvas, John Sidney, Morgane Rolland, Anais Valencia-Micolta, Sirinan Madnote, Alessandro Sette, Sorachai Nitayaphan, Punnee Pitisuttuthum, Jaranit Kaewkungwal, Supachai Rerks-Ngarm, Robert O'Connell, Nelson Michael, Merlin L. Robb, Mary Marovich, Jerome H. Kim

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody.

Original language	English
Article number	e0115582
Journal	PLoS ONE
Volume	10
Issue number	2
DOIs	https://doi.org/10.1371/journal.pone.0115582
State	Published - 10 Feb 2015
Externally published	Yes

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Ratto-Kim, S., De Souza, M. S., Currier, J. R., Karasavvas, N., Sidney, J., Rolland, M., Valencia-Micolta, A., Madnote, S., Sette, A., Nitayaphan, S., Pitisuttuthum, P., Kaewkungwal, J., Rerks-Ngarm, S., O'Connell, R., Michael, N., Robb, M. L., Marovich, M., & Kim, J. H. (2015). Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. PLoS ONE, 10(2), Article e0115582. https://doi.org/10.1371/journal.pone.0115582

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title = "Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E",

abstract = "We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody.",

author = "Silvia Ratto-Kim and {De Souza}, {Mark S.} and Currier, {Jeffrey R.} and Nicos Karasavvas and John Sidney and Morgane Rolland and Anais Valencia-Micolta and Sirinan Madnote and Alessandro Sette and Sorachai Nitayaphan and Punnee Pitisuttuthum and Jaranit Kaewkungwal and Supachai Rerks-Ngarm and Robert O'Connell and Nelson Michael and Robb, {Merlin L.} and Mary Marovich and Kim, {Jerome H.}",

note = "Funding Information: We thank the RV144 study participants. Gratitude is also expressed to Dr. Gustavo Kijak for helping with the class II analysis of these samples and Dr. Haitao Hu for helping with the manuscript editing. This work was supported by a cooperative agreement (W81XWH-11–2–0174) between the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., and the U.S. Army Medical Research and Materiel Command. ",

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Ratto-Kim, S, De Souza, MS, Currier, JR, Karasavvas, N, Sidney, J, Rolland, M, Valencia-Micolta, A, Madnote, S, Sette, A, Nitayaphan, S, Pitisuttuthum, P, Kaewkungwal, J, Rerks-Ngarm, S, O'Connell, R , Michael, N, Robb, ML, Marovich, M & Kim, JH 2015, 'Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E', PLoS ONE, vol. 10, no. 2, e0115582. https://doi.org/10.1371/journal.pone.0115582

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AU - Currier, Jeffrey R.

AU - Karasavvas, Nicos

AU - Sidney, John

AU - Rolland, Morgane

AU - Valencia-Micolta, Anais

AU - Madnote, Sirinan

AU - Sette, Alessandro

AU - Nitayaphan, Sorachai

AU - Pitisuttuthum, Punnee

AU - Kaewkungwal, Jaranit

AU - Rerks-Ngarm, Supachai

AU - O'Connell, Robert

AU - Michael, Nelson

AU - Robb, Merlin L.

AU - Marovich, Mary

AU - Kim, Jerome H.

N1 - Funding Information: We thank the RV144 study participants. Gratitude is also expressed to Dr. Gustavo Kijak for helping with the class II analysis of these samples and Dr. Haitao Hu for helping with the manuscript editing. This work was supported by a cooperative agreement (W81XWH-11–2–0174) between the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., and the U.S. Army Medical Research and Materiel Command.

PY - 2015/2/10

Y1 - 2015/2/10

N2 - We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody.

AB - We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody.

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Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E

Abstract

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