Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay

T. R. Burkot; F. Zavala; R. W. Gwadz; F. H. Collins; R. S. Nussenzweig; D. R. Roberts

doi:10.4269/ajtmh.1984.33.227

Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay

T. R. Burkot, F. Zavala, R. W. Gwadz, F. H. Collins, R. S. Nussenzweig, D. R. Roberts

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Abstract

A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.

Original language	English
Pages (from-to)	227-231
Number of pages	5
Journal	American Journal of Tropical Medicine and Hygiene
Volume	33
Issue number	2
DOIs	https://doi.org/10.4269/ajtmh.1984.33.227
State	Published - 1984

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10.4269/ajtmh.1984.33.227

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title = "Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay",

abstract = "A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.",

author = "Burkot, {T. R.} and F. Zavala and Gwadz, {R. W.} and Collins, {F. H.} and Nussenzweig, {R. S.} and Roberts, {D. R.}",

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T1 - Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay

AU - Burkot, T. R.

AU - Zavala, F.

AU - Gwadz, R. W.

AU - Collins, F. H.

AU - Nussenzweig, R. S.

AU - Roberts, D. R.

PY - 1984

Y1 - 1984

N2 - A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.

AB - A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.

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DO - 10.4269/ajtmh.1984.33.227

M3 - Article

AN - SCOPUS:0021247449

SN - 0002-9637

VL - 33

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EP - 231

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 2

ER -

Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay

Abstract

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