Identification of Plasmodium vivax sporozoites in mosquitoes using an enzyme-linked immunosorbent assay

R. A. Wirtz; T. R. Burkot; R. G. Andre; R. Rosenberg; W. E. Collins; D. R. Roberts

doi:10.4269/ajtmh.1985.34.1048

Identification of Plasmodium vivax sporozoites in mosquitoes using an enzyme-linked immunosorbent assay

R. A. Wirtz, T. R. Burkot, R. G. Andre, R. Rosenberg, W. E. Collins, D. R. Roberts

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Abstract

A sandwich enzyme-linked immunosorbent assay (ELISA) for identifying Plasmodium vivax sporozoites in mosquitoes is described. Monoclonal antibodies produced against Thailand P. vivax sporozoites were used in an ELISA to detect and identify homologous sporozoites of Southeast Asian, Mexican and North Korean origin in extracts of frozen or dried infected mosquitoes. The assay was sensitive enough to detect 1 infected mosquito in a pool of 20 insects or 125-250 sporozoites per 30 μl of mosquito extract. The use of a nonionic detergent and a single freeze-thaw to disrupt the circumsporozoite antigen significantly increased the sensitivity of the method.

Original language	English
Pages (from-to)	1048-1054
Number of pages	7
Journal	American Journal of Tropical Medicine and Hygiene
Volume	34
Issue number	6
DOIs	https://doi.org/10.4269/ajtmh.1985.34.1048
State	Published - 1985

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title = "Identification of Plasmodium vivax sporozoites in mosquitoes using an enzyme-linked immunosorbent assay",

abstract = "A sandwich enzyme-linked immunosorbent assay (ELISA) for identifying Plasmodium vivax sporozoites in mosquitoes is described. Monoclonal antibodies produced against Thailand P. vivax sporozoites were used in an ELISA to detect and identify homologous sporozoites of Southeast Asian, Mexican and North Korean origin in extracts of frozen or dried infected mosquitoes. The assay was sensitive enough to detect 1 infected mosquito in a pool of 20 insects or 125-250 sporozoites per 30 μl of mosquito extract. The use of a nonionic detergent and a single freeze-thaw to disrupt the circumsporozoite antigen significantly increased the sensitivity of the method.",

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T1 - Identification of Plasmodium vivax sporozoites in mosquitoes using an enzyme-linked immunosorbent assay

AU - Wirtz, R. A.

AU - Burkot, T. R.

AU - Andre, R. G.

AU - Rosenberg, R.

AU - Collins, W. E.

AU - Roberts, D. R.

PY - 1985

Y1 - 1985

N2 - A sandwich enzyme-linked immunosorbent assay (ELISA) for identifying Plasmodium vivax sporozoites in mosquitoes is described. Monoclonal antibodies produced against Thailand P. vivax sporozoites were used in an ELISA to detect and identify homologous sporozoites of Southeast Asian, Mexican and North Korean origin in extracts of frozen or dried infected mosquitoes. The assay was sensitive enough to detect 1 infected mosquito in a pool of 20 insects or 125-250 sporozoites per 30 μl of mosquito extract. The use of a nonionic detergent and a single freeze-thaw to disrupt the circumsporozoite antigen significantly increased the sensitivity of the method.

AB - A sandwich enzyme-linked immunosorbent assay (ELISA) for identifying Plasmodium vivax sporozoites in mosquitoes is described. Monoclonal antibodies produced against Thailand P. vivax sporozoites were used in an ELISA to detect and identify homologous sporozoites of Southeast Asian, Mexican and North Korean origin in extracts of frozen or dried infected mosquitoes. The assay was sensitive enough to detect 1 infected mosquito in a pool of 20 insects or 125-250 sporozoites per 30 μl of mosquito extract. The use of a nonionic detergent and a single freeze-thaw to disrupt the circumsporozoite antigen significantly increased the sensitivity of the method.

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U2 - 10.4269/ajtmh.1985.34.1048

DO - 10.4269/ajtmh.1985.34.1048

M3 - Article

AN - SCOPUS:0022375982

SN - 0002-9637

VL - 34

SP - 1048

EP - 1054

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 6

ER -

Identification of Plasmodium vivax sporozoites in mosquitoes using an enzyme-linked immunosorbent assay

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