Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope protein

K. Raviprakash; T. J. Kochel; D. Ewing; M. Simmons; I. Phillips; C. G. Hayes; K. R. Porter

doi:10.1016/S0264-410X(99)00570-8

Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope protein

K. Raviprakash^*, T. J. Kochel, D. Ewing, M. Simmons, I. Phillips, C. G. Hayes, K. R. Porter

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic in intradermally inoculated mice. The vaccinated mice produced dengue-1 specific antibodies that were both neutralizing and long lasting. Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E. Copyright (C) 2000 Elsevier Science Ltd.

Original language	English
Pages (from-to)	2426-2434
Number of pages	9
Journal	Vaccine
Volume	18
Issue number	22
DOIs	https://doi.org/10.1016/S0264-410X(99)00570-8
State	Published - May 2000
Externally published	Yes

Keywords

Antibody response
DNA vaccine
Dengue virus
Mouse

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10.1016/S0264-410X(99)00570-8

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title = "Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope protein",

abstract = "Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic in intradermally inoculated mice. The vaccinated mice produced dengue-1 specific antibodies that were both neutralizing and long lasting. Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E. Copyright (C) 2000 Elsevier Science Ltd.",

keywords = "Antibody response, DNA vaccine, Dengue virus, Mouse",

author = "K. Raviprakash and Kochel, {T. J.} and D. Ewing and M. Simmons and I. Phillips and Hayes, {C. G.} and Porter, {K. R.}",

note = "Funding Information: This work was supported by the US Naval Medical Research and Development Command work unit 61102A M00101 1 443. The experiments reported herein were conducted according to the principles set forth in the “Guide for the care and use of laboratory animals”, Institute of Laboratory Animal Resources, National Research Council, National Academy Press, 1996. ",

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doi = "10.1016/S0264-410X(99)00570-8",

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AU - Raviprakash, K.

AU - Kochel, T. J.

AU - Ewing, D.

AU - Simmons, M.

AU - Phillips, I.

AU - Hayes, C. G.

AU - Porter, K. R.

N1 - Funding Information: This work was supported by the US Naval Medical Research and Development Command work unit 61102A M00101 1 443. The experiments reported herein were conducted according to the principles set forth in the “Guide for the care and use of laboratory animals”, Institute of Laboratory Animal Resources, National Research Council, National Academy Press, 1996.

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N2 - Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic in intradermally inoculated mice. The vaccinated mice produced dengue-1 specific antibodies that were both neutralizing and long lasting. Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E. Copyright (C) 2000 Elsevier Science Ltd.

AB - Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic in intradermally inoculated mice. The vaccinated mice produced dengue-1 specific antibodies that were both neutralizing and long lasting. Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E. Copyright (C) 2000 Elsevier Science Ltd.

KW - Antibody response

KW - DNA vaccine

KW - Dengue virus

KW - Mouse

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JO - Vaccine

JF - Vaccine

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ER -

Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope protein

Abstract

Keywords

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