Immunohistochemical expression of π-class glutathione S-transferase is down-regulated in adenocarcinoma of the prostate

Christopher A. Moskaluk, Paul H. Duray, Kenneth H. Cowan, Marston Linehan, Maria J. Merino*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


BACKGROUND. Glutathione S-transferase is often up-regulated in neoplastic tissues. A single previous study found a loss of expression associated with carcinogenesis of the prostate. METHODS. To extend these results, the authors performed immunohistochemical staining for the π-class of glutathione S-transferase (GSTπ) on 74 archival sequential prostate specimens. The antibody used was derived from rabbits immunized against purified human GSTπ. Paraffin blocks containing both benign tissue and adenocarcinoma were studied. RESULTS. Heterogeneous expression of GSTπ in benign acini was found in 96% of cases, but GSTπ was not expressed in 95% of invasive adenocarcinomas of the prostate, nor was it expressed in any of the loci of high grade prostatic intraepithelial neoplasia. Basal cells of benign acini showed strong, diffuse staining for GSTπ, whereas the secretory luminal epithelium expressed GSTπ weakly and locally. CONCLUSIONS. This study confirms the down-regulation of GSTπ in adenocarcinoma of the prostate and shows that the loss of GSTπ expression is a phenotype associated with malignant transformation.

Original languageEnglish
Pages (from-to)1595-1599
Number of pages5
Issue number8
StatePublished - 15 Apr 1997
Externally publishedYes


  • glutathione S-transferase
  • immunohistochemistry
  • prostate carcinoma
  • prostatic intraepithelial neoplasia


Dive into the research topics of 'Immunohistochemical expression of π-class glutathione S-transferase is down-regulated in adenocarcinoma of the prostate'. Together they form a unique fingerprint.

Cite this