TY - JOUR
T1 - Immunohistochemical expression of π-class glutathione S-transferase is down-regulated in adenocarcinoma of the prostate
AU - Moskaluk, Christopher A.
AU - Duray, Paul H.
AU - Cowan, Kenneth H.
AU - Linehan, Marston
AU - Merino, Maria J.
PY - 1997/4/15
Y1 - 1997/4/15
N2 - BACKGROUND. Glutathione S-transferase is often up-regulated in neoplastic tissues. A single previous study found a loss of expression associated with carcinogenesis of the prostate. METHODS. To extend these results, the authors performed immunohistochemical staining for the π-class of glutathione S-transferase (GSTπ) on 74 archival sequential prostate specimens. The antibody used was derived from rabbits immunized against purified human GSTπ. Paraffin blocks containing both benign tissue and adenocarcinoma were studied. RESULTS. Heterogeneous expression of GSTπ in benign acini was found in 96% of cases, but GSTπ was not expressed in 95% of invasive adenocarcinomas of the prostate, nor was it expressed in any of the loci of high grade prostatic intraepithelial neoplasia. Basal cells of benign acini showed strong, diffuse staining for GSTπ, whereas the secretory luminal epithelium expressed GSTπ weakly and locally. CONCLUSIONS. This study confirms the down-regulation of GSTπ in adenocarcinoma of the prostate and shows that the loss of GSTπ expression is a phenotype associated with malignant transformation.
AB - BACKGROUND. Glutathione S-transferase is often up-regulated in neoplastic tissues. A single previous study found a loss of expression associated with carcinogenesis of the prostate. METHODS. To extend these results, the authors performed immunohistochemical staining for the π-class of glutathione S-transferase (GSTπ) on 74 archival sequential prostate specimens. The antibody used was derived from rabbits immunized against purified human GSTπ. Paraffin blocks containing both benign tissue and adenocarcinoma were studied. RESULTS. Heterogeneous expression of GSTπ in benign acini was found in 96% of cases, but GSTπ was not expressed in 95% of invasive adenocarcinomas of the prostate, nor was it expressed in any of the loci of high grade prostatic intraepithelial neoplasia. Basal cells of benign acini showed strong, diffuse staining for GSTπ, whereas the secretory luminal epithelium expressed GSTπ weakly and locally. CONCLUSIONS. This study confirms the down-regulation of GSTπ in adenocarcinoma of the prostate and shows that the loss of GSTπ expression is a phenotype associated with malignant transformation.
KW - glutathione S-transferase
KW - immunohistochemistry
KW - prostate carcinoma
KW - prostatic intraepithelial neoplasia
UR - http://www.scopus.com/inward/record.url?scp=0030900216&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0142(19970415)79:8<1595::AID-CNCR23>3.0.CO;2-S
DO - 10.1002/(SICI)1097-0142(19970415)79:8<1595::AID-CNCR23>3.0.CO;2-S
M3 - Article
C2 - 9118044
AN - SCOPUS:0030900216
SN - 0008-543X
VL - 79
SP - 1595
EP - 1599
JO - Cancer
JF - Cancer
IS - 8
ER -