In situ expression of cagA and risk of gastroduodenal disease in helicobacter pylori-infected children

James R. Rick, Matthew Goldman, Cristina Semino-Mora, Hui Liu, Cara Olsen, Eugenia Rueda-Pedraza, Carolyn Sullivan, Andre Dubois*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Background and Aim: Gastroduodenal disease is more common among adults and children with cagA+ Helicobacter pylori infection, but disease severity varies among those infected with cagA+ strains. We examined whether cagA in situ expression can predict disease manifestations among H pylori-infected children. Patients and Methods: Fifty-one children were selected from 805 patients with abdominal symptoms who underwent esophagogastroduodenoscopy with gastric biopsies. Endoscopic and histologic gastritis were scored and H pylori colonization was quantified by Genta stain and in situ hybridization expression of 16S rRNA and cagA. Results: Endoscopy was either normal (n = 14) or demonstrated nodularity (n = 18), gastric ulcer (n = 8) or duodenal ulcer (n = 11). H pylori was present in 7, 18, 6, and 10 children, respectively. Expression of 16S rRNA and cagA were significantly higher in children with ulcer compared with normal children. The fraction of H pylori bacteria expressing cagA in situ was higher in children with ulcer compared to those with endoscopic nodularity (P < 0.05). Conclusions: Thus, cagA in situ expression is increased in H pylori-infected children with peptic ulcers and may play a role in the pathogenesis of peptic ulcer disease during childhood. Determination of in situ expression of cagA complements traditional isolation and in vitro testing of single-colony isolates.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalJournal of Pediatric Gastroenterology and Nutrition
Issue number2
StatePublished - Feb 2010
Externally publishedYes


  • CagA gene expression
  • Duodenal ulcer
  • Gastric ulcer
  • Gastritis
  • H pylori
  • Nodularity
  • Virulence factor


Dive into the research topics of 'In situ expression of cagA and risk of gastroduodenal disease in helicobacter pylori-infected children'. Together they form a unique fingerprint.

Cite this