In vitro adipose tissue engineering using an electrospun nanofibrous scaffold

Rabie M. Shanti, Sasa Janjanin, Wan Ju Li, Leon J. Nesti, Michael B. Mueller, Megan B. Tzeng, Rocky S. Tuan

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Electrospun 3-dimensional nanofibrous scaffolds share morphologic similarities to collagen fibrils, and promote favorable biologic responses of seeded cells. In this study, we have fabricated a 3-dimensional nanofibrous scaffold made of poly L-lactic acid, and examined its ability to support and maintain the adipogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro. After a 21-day incubation, oil red O staining of constructs treated with adipogenic supplements revealed positive adipose-like staining, compared with lack of staining in untreated cultures. Semi-quantitative RT-PCR analysis of human bone marrow-derived mesenchymal stem cells cultured in adipogenic medium revealed highly elevated levels of adipogenesis-associated genes (1797-fold for lipoprotein lipase, and 5.6-fold for peroxisome proliferator-activated receptor γ). Immunofluorescence staining of cellular constructs in adipogenic culture media showed the presence of lipoprotein lipase vesicles, a characteristic feature of adipose tissue. These results suggest that the poly L-lactic acid-based nanofibrous scaffold is a promising candidate for adult stem cell-based engineering of adipose tissue.

Original languageEnglish
Pages (from-to)566-571
Number of pages6
JournalAnnals of Plastic Surgery
Issue number5
StatePublished - Nov 2008
Externally publishedYes


  • Adipose tissue engineering
  • Electrospinning
  • Mesechymal stem cells
  • Nanofibers
  • Tissue engineering


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