TY - JOUR
T1 - In vitro comparison of human fibroblasts from intact and ruptured ACL for use in tissue engineering
AU - Brune, Thierry
AU - Borel, A.
AU - Gilbert, T. W.
AU - Franceschi, J. P.
AU - Badylak, S. F.
AU - Sommer, P.
PY - 2007
Y1 - 2007
N2 - The present study compares fibroblasts extracted from intact and ruptured human anterior cruciate ligaments (ACL) for creation of a tissue engineered ACL-construct, made of porcine small intestinal submucosal extracellular matrix (SIS-ECM) seeded with these ACL cells. The comparison is based on histological, immunohistochemical and RT-PCR analyses. Differences were observed between cells in a ruptured ACL (rACL) and cells in an intact ACL (iACL), particularly with regard to the expression of integrin subunits and smooth muscle actin (SMA). Despite these differences in the cell source, both cell populations behaved similarly when seeded on an SIS-ECM scaffold, with similar cell morphology, connective tissue organization and composition, SMA and integrin expression. This study shows the usefulness of naturally occurring scaffolds such as SIS-ECM for the study of cell behaviour in vitro, and illustrates the possibility to use autologous cells extracted from ruptured ACL biopsies as a source for tissue engineered ACL constructs.
AB - The present study compares fibroblasts extracted from intact and ruptured human anterior cruciate ligaments (ACL) for creation of a tissue engineered ACL-construct, made of porcine small intestinal submucosal extracellular matrix (SIS-ECM) seeded with these ACL cells. The comparison is based on histological, immunohistochemical and RT-PCR analyses. Differences were observed between cells in a ruptured ACL (rACL) and cells in an intact ACL (iACL), particularly with regard to the expression of integrin subunits and smooth muscle actin (SMA). Despite these differences in the cell source, both cell populations behaved similarly when seeded on an SIS-ECM scaffold, with similar cell morphology, connective tissue organization and composition, SMA and integrin expression. This study shows the usefulness of naturally occurring scaffolds such as SIS-ECM for the study of cell behaviour in vitro, and illustrates the possibility to use autologous cells extracted from ruptured ACL biopsies as a source for tissue engineered ACL constructs.
KW - Anterior cruciate ligament
KW - Elastic network
KW - Extracted fibroblasts
KW - In vitro models
KW - Integrins
KW - Small intestinal submucosa scaffold
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=38549100664&partnerID=8YFLogxK
U2 - 10.22203/ecm.v014a08
DO - 10.22203/ecm.v014a08
M3 - Article
C2 - 18085506
AN - SCOPUS:38549100664
SN - 1473-2262
VL - 14
SP - 78
EP - 90
JO - European Cells and Materials
JF - European Cells and Materials
ER -