In vivo assessment of a biological occluder for notes gastrotomy closure

Alejandro F. Sanz; Toshitaka Hoppo; Bart P. Witteman; Bryan N. Brown; Thomas W. Gilbert; Stephen F. Badylak; Blair A. Jobe; Alejandro Nieponice

doi:10.1097/SLE.0b013e3182a1c31b

In vivo assessment of a biological occluder for notes gastrotomy closure

Alejandro F. Sanz, Toshitaka Hoppo, Bart P. Witteman, Bryan N. Brown, Thomas W. Gilbert, Stephen F. Badylak, Blair A. Jobe, Alejandro Nieponice^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Background: The objective of this study is to evaluate the closure of a transgastric natural orifice transluminal endoscopic surgery (NOTES) access using a multilayer extracellular matrix (ECM) occluder in a canine model. Materials And Methods: Mongrel dogs (n=4) underwent a transgastric NOTES peritoneoscopy and the gastrotomy was closed by deploying a 2-sided ECM occluder. Animals were killed at 7 days (n=2) and 8 weeks (n=2) for macroscopic and microscopic assessment. Results: All procedures were completed without any complications. No air leaks were detected immediately after the procedure and at 48 hours postoperatively. At 7 days, ECM appeared to be resolved and mild mucosal inflammation was found at the site of gastrotomy. At 8 weeks, the gastrotomy site was macroscopically and microscopically covered with a normal-appearing gastric mucosa. There was an absence of inflammatory cells and no evidence of the ECM. Conclusion: The ECM occluder is safe and effective in this "proof-of-concept" preclinical model.

Original language	English
Pages (from-to)	322-326
Number of pages	5
Journal	Surgical Laparoscopy, Endoscopy and Percutaneous Techniques
Volume	24
Issue number	4
DOIs	https://doi.org/10.1097/SLE.0b013e3182a1c31b
State	Published - Aug 2014
Externally published	Yes

Keywords

Canine model
Gastrotomy
Inflammatory cells
Multilayer extracellular matrix accluder
Notes
Peritoneoscopy

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10.1097/SLE.0b013e3182a1c31b

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title = "In vivo assessment of a biological occluder for notes gastrotomy closure",

abstract = "Background: The objective of this study is to evaluate the closure of a transgastric natural orifice transluminal endoscopic surgery (NOTES) access using a multilayer extracellular matrix (ECM) occluder in a canine model. Materials And Methods: Mongrel dogs (n=4) underwent a transgastric NOTES peritoneoscopy and the gastrotomy was closed by deploying a 2-sided ECM occluder. Animals were killed at 7 days (n=2) and 8 weeks (n=2) for macroscopic and microscopic assessment. Results: All procedures were completed without any complications. No air leaks were detected immediately after the procedure and at 48 hours postoperatively. At 7 days, ECM appeared to be resolved and mild mucosal inflammation was found at the site of gastrotomy. At 8 weeks, the gastrotomy site was macroscopically and microscopically covered with a normal-appearing gastric mucosa. There was an absence of inflammatory cells and no evidence of the ECM. Conclusion: The ECM occluder is safe and effective in this {"}proof-of-concept{"} preclinical model.",

keywords = "Canine model, Gastrotomy, Inflammatory cells, Multilayer extracellular matrix accluder, Notes, Peritoneoscopy",

author = "Sanz, {Alejandro F.} and Toshitaka Hoppo and Witteman, {Bart P.} and Brown, {Bryan N.} and Gilbert, {Thomas W.} and Badylak, {Stephen F.} and Jobe, {Blair A.} and Alejandro Nieponice",

year = "2014",

month = aug,

doi = "10.1097/SLE.0b013e3182a1c31b",

language = "English",

volume = "24",

pages = "322--326",

journal = "Surgical Laparoscopy, Endoscopy and Percutaneous Techniques",

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T1 - In vivo assessment of a biological occluder for notes gastrotomy closure

AU - Sanz, Alejandro F.

AU - Hoppo, Toshitaka

AU - Witteman, Bart P.

AU - Brown, Bryan N.

AU - Gilbert, Thomas W.

AU - Badylak, Stephen F.

AU - Jobe, Blair A.

AU - Nieponice, Alejandro

PY - 2014/8

Y1 - 2014/8

N2 - Background: The objective of this study is to evaluate the closure of a transgastric natural orifice transluminal endoscopic surgery (NOTES) access using a multilayer extracellular matrix (ECM) occluder in a canine model. Materials And Methods: Mongrel dogs (n=4) underwent a transgastric NOTES peritoneoscopy and the gastrotomy was closed by deploying a 2-sided ECM occluder. Animals were killed at 7 days (n=2) and 8 weeks (n=2) for macroscopic and microscopic assessment. Results: All procedures were completed without any complications. No air leaks were detected immediately after the procedure and at 48 hours postoperatively. At 7 days, ECM appeared to be resolved and mild mucosal inflammation was found at the site of gastrotomy. At 8 weeks, the gastrotomy site was macroscopically and microscopically covered with a normal-appearing gastric mucosa. There was an absence of inflammatory cells and no evidence of the ECM. Conclusion: The ECM occluder is safe and effective in this "proof-of-concept" preclinical model.

AB - Background: The objective of this study is to evaluate the closure of a transgastric natural orifice transluminal endoscopic surgery (NOTES) access using a multilayer extracellular matrix (ECM) occluder in a canine model. Materials And Methods: Mongrel dogs (n=4) underwent a transgastric NOTES peritoneoscopy and the gastrotomy was closed by deploying a 2-sided ECM occluder. Animals were killed at 7 days (n=2) and 8 weeks (n=2) for macroscopic and microscopic assessment. Results: All procedures were completed without any complications. No air leaks were detected immediately after the procedure and at 48 hours postoperatively. At 7 days, ECM appeared to be resolved and mild mucosal inflammation was found at the site of gastrotomy. At 8 weeks, the gastrotomy site was macroscopically and microscopically covered with a normal-appearing gastric mucosa. There was an absence of inflammatory cells and no evidence of the ECM. Conclusion: The ECM occluder is safe and effective in this "proof-of-concept" preclinical model.

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KW - Inflammatory cells

KW - Multilayer extracellular matrix accluder

KW - Notes

KW - Peritoneoscopy

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JF - Surgical Laparoscopy, Endoscopy and Percutaneous Techniques

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In vivo assessment of a biological occluder for notes gastrotomy closure

Abstract

Keywords

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