In vivo fitness adaptations of colistin-resistant Acinetobacter baumannii isolates to oxidative stress

Crystal L. Jones; Shweta S. Singh; Yonas Alamneh; Leila G. Casella; Robert K. Ernst; Emil P. Lesho; Paige E. Waterman; Daniel V. Zurawski

doi:10.1128/AAC.00598-16

In vivo fitness adaptations of colistin-resistant Acinetobacter baumannii isolates to oxidative stress

Crystal L. Jones^*, Shweta S. Singh, Yonas Alamneh, Leila G. Casella, Robert K. Ernst, Emil P. Lesho, Paige E. Waterman, Daniel V. Zurawski

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The loss of fitness in colistin-resistant (CR) Acinetobacter baumannii was investigated using longitudinal isolates from the same patient. Early CR isolates were outcompeted by late CR isolates for growth in broth and survival in the lungs of mice. Fitness loss was associated with an increased susceptibility to oxidative stress since early CR strains had reduced in vitro survival in the presence of hydrogen peroxide and decreased catalase activity compared to that of late CR and colistinsusceptible (CS) strains.

Original language	English
Article number	e00598
Journal	Antimicrobial Agents and Chemotherapy
Volume	61
Issue number	3
DOIs	https://doi.org/10.1128/AAC.00598-16
State	Published - 1 Mar 2017
Externally published	Yes

Keywords

Acinetobacter
Catalase
ESKAPE
Fitness
Mass spectrometry
Mouse model
Serial isolation
Virulence

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title = "In vivo fitness adaptations of colistin-resistant Acinetobacter baumannii isolates to oxidative stress",

abstract = "The loss of fitness in colistin-resistant (CR) Acinetobacter baumannii was investigated using longitudinal isolates from the same patient. Early CR isolates were outcompeted by late CR isolates for growth in broth and survival in the lungs of mice. Fitness loss was associated with an increased susceptibility to oxidative stress since early CR strains had reduced in vitro survival in the presence of hydrogen peroxide and decreased catalase activity compared to that of late CR and colistinsusceptible (CS) strains.",

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author = "Jones, {Crystal L.} and Singh, {Shweta S.} and Yonas Alamneh and Casella, {Leila G.} and Ernst, {Robert K.} and Lesho, {Emil P.} and Waterman, {Paige E.} and Zurawski, {Daniel V.}",

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AU - Jones, Crystal L.

AU - Singh, Shweta S.

AU - Alamneh, Yonas

AU - Casella, Leila G.

AU - Ernst, Robert K.

AU - Lesho, Emil P.

AU - Waterman, Paige E.

AU - Zurawski, Daniel V.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - The loss of fitness in colistin-resistant (CR) Acinetobacter baumannii was investigated using longitudinal isolates from the same patient. Early CR isolates were outcompeted by late CR isolates for growth in broth and survival in the lungs of mice. Fitness loss was associated with an increased susceptibility to oxidative stress since early CR strains had reduced in vitro survival in the presence of hydrogen peroxide and decreased catalase activity compared to that of late CR and colistinsusceptible (CS) strains.

AB - The loss of fitness in colistin-resistant (CR) Acinetobacter baumannii was investigated using longitudinal isolates from the same patient. Early CR isolates were outcompeted by late CR isolates for growth in broth and survival in the lungs of mice. Fitness loss was associated with an increased susceptibility to oxidative stress since early CR strains had reduced in vitro survival in the presence of hydrogen peroxide and decreased catalase activity compared to that of late CR and colistinsusceptible (CS) strains.

KW - Acinetobacter

KW - Catalase

KW - ESKAPE

KW - Fitness

KW - Mass spectrometry

KW - Mouse model

KW - Serial isolation

KW - Virulence

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SN - 0066-4804

VL - 61

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 3

M1 - e00598

ER -

In vivo fitness adaptations of colistin-resistant Acinetobacter baumannii isolates to oxidative stress

Abstract

Keywords

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