Increased CO2 stimulates K/Rb reabsorption mediated by H-K-ATPase in CCD of potassium-restricted rabbit

X. Zhou, S. Nakamura, S. L. Xia, C. S. Wingo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Apical H-K-ATPase in the cortical collecting duct (CCD) plays an important role in urinary acidification and K reabsorption. Our previous studies demonstrated that an H-K-ATPase mediates, in part, Rb reabsorption in rabbit CCD (Zhou X and Wingo CS. Am J Physiol Renal Fluid Electrolyte Physiol 263: F1134-F1141, 1992). The purpose of these experiments was to examine using in vitro microperfused CCD from K-restricted rabbits 1) whether an acute increase in PCO2 and, presumably, intracellular acidosis stimulate K absorptive flux; and 2) whether this stimulation was dependent on the presence of a functional H-K-ATPase. Rb reabsorption was significantly increased after exposure to 10% CO2 in CCD, and this effect was persistent for the entire 10% CO2 period, whereas 10 μM SCH-28080 in the perfusate totally abolished the stimulation of Rb reabsorption by 10% CO2. After stimulation of Rb reabsorption by 10% CO2, subsequent addition of 0.1 mM methazolamide, an inhibitor of carbonic anhydrase, failed to affect Rb reabsorption. However, simultaneous exposure to 10% CO2 and methazolamide prevented the stimulation of Rb reabsorption. Treatment with the intracellular calcium chelator MAPTAM (0.5 μM) inhibited the stimulation of Rb reabsorption by 10% CO2. Similar inhibition was also observed in the presence of either a calmodulin inhibitor, W-7 (0.5 μM), or colchicine (0.5 mM), an inhibitor of tubulin polymerization. In time control studies, the perfusion time did not significantly affect Rb reabsorption. We conclude the following: 1) stimulation of Rb reabsorption on exposure to 10% CO2 is dependent on the presence of a functional H-K-ATPase and appears to be regulated in part by the insertion of this enzyme into the apical plasma membrane by exocytosis; 2) insertion of H-K-ATPase requires changes in intracellular pH and needs a basal level of intracellular calcium concentration; and 3) H-K-ATPase insertion occurs by a microtubule-dependent process.

Original languageEnglish
Pages (from-to)F366-F373
JournalAmerican Journal of Physiology - Renal Physiology
Volume281
Issue number2 50-2
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Calcium
  • Calmodulin
  • Cortical collecting duct
  • Exocytosis
  • Intracellular pH
  • Microtubules

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