Inducible cytosolic enzyme activity for the production of nitrogen oxides from L-arginine in hepatocytes

T. R. Billiar*, R. D. Curran, D. J. Stuehr, J. Stadler, R. L. Simmons, S. A. Murray

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

174 Scopus citations

Abstract

The in vivo conditions needed for the induction of nitrogen oxide synthesis by hepatocytes were determined. Hepatocytes obtained from rats injected with killed Corynebacterium parvum spontaneously produced NO2-+NO3- in culture and were found to contain cytosolic enzyme activity for nitrogen oxide synthesis. The enzyme activity required both L-arginine and NADPH, and was not found in hepatocytes obtained from normal rats or rats injected with lipopoly-saccharide (LPS) alone. In contrast, nonparenchymal cells were stimulated to synthesize NO2-+NO3- by LPS. These results show the presence of inducible cytosolic enzyme activity for nitrogen oxide synthesis in hepatocytes, which is distinct from nonparenchymal cell NO- synthesis.

Original languageEnglish
Pages (from-to)1034-1040
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume168
Issue number3
DOIs
StatePublished - 16 May 1990
Externally publishedYes

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